do's and dont's in the lab

1. Clinical Laboratory’s Do’s and Don t’s BSMLS 2D 2013-2014

2. MEMBERS : Bautista, Charles Christian H. Brioso , Princess Michelle Cabinian,Jaymilu Cabral, Teresa Liway A. Garcia , Maria Karissa A. Gonzales, Jemmuel E. Lontoc, Shelah Mae H. Macatangay , Sophia Tatlonghari , Edmon H. Villacorta , Jon Vincent B .

3. Lab Safety

4. Why does it matter? Safe working protects: You Other lab workers Cleaners Visitors Your work

5. Protecting yourself (PPE) Fasten laboratory coats Don’t wear sandals or open shoes Tie back long hair

6. Gloves Use the correct ones for the job you will be doing Remove gloves before using instruments, telephone, and leaving the laboratory

7. Laboratory hygiene Do not eat , drink or smoke in a laboratory Do not apply cosmetics Do not touch your face, mouth or eyes Do not suck pens or chew pencils

8. Glassware Safety Do not dispose broken glassware in a regular trashcan . When pouring liquids into glassware, make sure the container you are pouring into is resting on a table at least a hands breadth from the edge.

9. Spillages Clear up spillage promptly Dispose of any hazardous material as toxic waste “Messy workers are usually poor workers! !”

10. Electrical Hazard Always do a visual check on electrical equipment before use, look for obvious wear or defects

11. Waste Disposal Do not put materials down the drain or in with normal waste unless authorised to do so Wear appropriate PPE when handling clinical waste

12. Chemical Safety Always add acid to water Pour from large containers to smaller ones. Do not taste any chemical. Do not put your nose over the container and inhale the fumes. Acid Water

13. Fire Hazard Use the smallest quantities of flammable solvents practicable. Do not use a Bunsen burner in any area where flammable solvents are handled.

14. Risk of Infection If exposed to a bio hazardous specimen: Immediately wash the area with soap and H 2 O Flush splashes to nose, mouth or skin with H 2 O Irrigate eyes with clean H 2 O or saline Perform background tests

15. Aseptic Technique Perform proper hand washing

16. Reading Volume Measurements All readings must be made with the eye on level with the meniscus to avoid parallax error.

17. Pipetting techniques Hold the pipet between the thumb and forefinger Do not pipet using your mouth, use a rubber aspirator

18. Operating a Centrifuge Do not remove the cover of the centrifuge until it has come to a complete stop Make sure the loads are balanced

19. U sing microscope Gently wipe the lens with lens paper or muslin cloth Always carry the microscope with one hand supporting the base and the other hand around the arm.

20. Sharps Hazards Always use sterile,disposable needle Never recap a needle Dispose sharps in puncture-resistant, leak-proof container

21. Order of Draw and Inversion Always follow the correct order of draw of tubes and tube inversion Purple(8x) Blue(3-4x) Red glass (0x) Red plastic (5x)

22. Syringes Always transfer immediately blood drawn from syringe into an evacuated tube

23. Transporting samples to the laboratory Deliver samples to the laboratory within 45 minutes and centrifuge on arrival

24. Specimen Collection and Handling Procedures Allow samples to clot fully before centrifugation to complete separation of the cells and serum Protect bilirubin, Vit A and carotene samples from light

25. Laboratory Cleanliness Do maintain good lab cleanliness - it ’ s for your own safety and as well as for others!

26. Thank you!