Electron Transport Chain_Aras Beauty_ppt.ppt

Eletron Transport Chain is a fundamental process of metabolism in our body.

1. Electron Transport and
Oxidative Phosphorylation
By Aras Beauty

2. Mitochondria
•outer membrane relatively permeable
•inner membrane semi permeable
•Compartmentalization

3. Most energy from Redox
•electrons during metabolic reactions sent to NAD and FAD
–Glycolysis
–Pyruvate dehydrogenase reaction
–Krebs

4. Electron Transport Chain

5. 4 Complexes
•proteins in specific order
•Transfers 2 electrons in specific order
–Proteins localized in complexes
–Electrons ultimately reduce oxygen to water

6. Complex 1
•Has NADH binding site
–NADH reductase activity
–NADH ---> FMN--->FeS---> ubiquinone
–ubiquinone ---> ubiquinone H
2
–4 H
+
pumped/NADH

7. Complex II
•succinate ---FAD—ubiquinone
–Contains coenzyme Q
–FADH
2binding site

8. Complex III
•ubiquinone -ubiquinone
ox
•while cyt C gets reduced
•Also contains cytochromes b

9. Complex IV
•reduction of oxygen
•cytochrome oxidase
•cyt a+a3
red---> oxidized state
•oxygen ---> water
•Pumps 2H
+
out

10. Totals
•Proton gradient created as electrons
transferred to oxygen forming water
–10 H
+
/ NADH
–6 H
+
/ FADH
2

11. Totals
NADH
10 H
+
X 1 ATP= 2.5 ATP
4 H
+
FADH
2
6 H
+
X 1 ATP= 1.5 ATP
4 H
+

12. Total ATP from mitochondrial
matrix
•Pyruvate dehydrogenase
–NADH ……………………………….2.5 ATP
•Krebs
–3 NADH X 2.5 ATP/NADH ……….7.5 ATP
–FADH
2X 1.5 ATP / FADH
2……….1.5 ATP
–GTP X 1 ATP / GTP ……………..1.0 ATP
(from a separate reaction)
Total…………….12.5 ATP
(Per glucose = X 2 = 25 ATP)

13. THANKYOU 