embryo_culture_and_its_application_plant_tissueculture.pptx

plant tissue culture technique for embryo culture

1. EMBRYO CULTURE AND ITS APPLICATION

2. EMBRYO CULTURE Embryo culture is a type of Tissue Culture technique. WHAT IS EMBRYO ? Male gamete + Female gamete (fusion) f e rt i l i z a t i on zygote develop embryo

3. WHAT IS EMBRYO CULTURE ? Deals with sterile isolation and In- vitro growth of zygotic immature/mature embryo into a plantlet. In Somatic Embryogenesis technique we develop the embryo into the plant with the help of Explant. The part we select out of an living organism from which we make the whole plant is called as Explant.

4. METHODS OF EMBRYO CULTURE methods of embryo culture Mature embryo culture Immature embryo culture OR Embryo rescue method

5. MATURE EMBRYO CULTURE The development stage is gained by embryo. Isolation of mature embryo from ripe seeds. Used when embryo doesn’t survives in-vivo(because of seed dormancy chemical inhibitors or mechanical resistance).

6. IMMATURE EMBRYO CULTURE/ EMBRYO RESCUE METHOD It is still in the development stage. Isolation of immature embryo from unripe/hybrid/sterile seed which fails to develop. Used to rescue embryo of wide crosses or distant hybridization (inter-specific and inter-generic cross)

7. EMBRYO RESCUE METHOD

8. PROCEDURE OF EMBRYO CULTURE Pluck or take developed fruit Surface sterilize with 0.01% tween 20 for 15 minutes followed by washing with distilled water Sterilize with 0.01% mercuric chloride (HgCl2) for 10-15 minutes, followed by washing with distilled water Break seed aseptically and isolate the embryo Inoculate embryo on callus proliferating media After four weeks of inoculation callus appears. After 4 weeks, green callus and embryoids starts appearing And after 8 weeks of inoculation, subculture on shoot regenerating medium is to be done. After 12 weeks shoot appears.

9. APPLICATIONS OF EMBRYOCULTURE Production of plant from embryo. Study the relationship between embryo and endosperm. Prevents Embryo abortion. Overcome seed dormancy. Sterile seed due to early ripening e.g. Peach, cherry, apricot and plum. Used in scientific study as well Clonal propagation

10. Embryo endosperm transplant. Haploid production. Biotechnology. Shortening of breeding cycle.