Flame Photometric Estimation

Flame Photometric Estimation of Sodium and Potassium
Ion Present In Water Sample of Darna and Godavari
River...


• Flame Photometry also known as flame atomic emission spectrometry is a branch of analytical science that examined the species in form of an atom, mostly it works on the principle of ionization of alkali metal salt drawn into a non-luminous flame
• Alkali metal salt absorbs energy from flame and emits the light of a characteristic wavelength which is observed by the change in intensity of color, the energy absorbed was enough to vaporize alkali metal salt.
• In the present article the comparative studies on sodium and potassium content present in a water sample collected from Darna and Godavari River were calculated.

This Presentation Contains the following...
#Information about Paper
#Abstract
#Materials
#Method
#Characteristic Wavelengths of The Elements
#Procedure
#Result and Discussion
#Conclusion
#References

Thanks for the Help and Guidance of Dr. P. B. Choudhari (Assistant Professor, Pharmaceutical Chemistry) and Mr. V. A. Thorat (Assistant Professor, Department of Pharmacognosy) of Bharati Vidyapeeth College of Pharmacy, Kolhapur.

1. Flame Photometric Estimation of Sodium and Potassium
Ion Present In Water Sample of Darnaand Godavari
River
Presented by:-
Mr. Pritam P. Kolge
M. Pharm 2
nd
Year Sem IV
Department of Pharmaceutical Quality
Assurance
1
Journal Club Faculty:-
Mr. V. A. Thorat
Assistant Professor,
Department of Pharmacognosy
Guide:-
Dr.P. B. Choudhari
Assistant Professor,
Department of Pharmaceutical Chemistry
Bharati Vidyapeeth College of Pharmacy, Kolhapur
26/7/2021

2. Information about Paper
•Authors-HemantU.Chikhale,PratibhaU.Chikhale
•Journal-InternationalJournalofScientific&EngineeringResearch
•AcceptedDate-January-2017
2

3. •FlamePhotometryalsoknownasflameatomicemissionspectrometryisabranchof
analyticalsciencethatexaminedthespeciesinformofatom,mostlyitworkson
principleofionizationofalkalimetalsaltdrawnintoanon-luminousflame.
•Alkalimetalsaltabsorbenergyfromflameandemitthelightofcharacteristic
wavelengthwhichisobservedbychangeinintensityofcolor,theenergyabsorbwas
enoughtovaporizealkalimetalsalt.
•Inpresentarticlethecomparativestudiesonsodiumandpotassiumcontentpresentin
watersamplecollectedfromDarnaandGodavari(Nashik)Riverwerecalculated.
Abstract
3

4. •Material:SodiumChloride(AR),PotassiumChloride(AR),Distilledwater,Water
samplecollectedfromDarnaandGodavariRiver.
•Instrument:Equiptronics-EQ-850A/855Amanufactureby-EquiptronicsIndia
pvt.ltd.
•Apparatus:Volumetricflasks,beaker,Glassrod.
Materials
4

5. FlamePhotometricMethod
•Preparationofstandardsolution:
1)Ananalyticalreagentqualitysodiumchloride(NaCl)isweighabout2.542
gmandtransfersitinto1litervolumetricflaskthroughafunnel.
Simultaneouslyweigh1.909gmofanalyticalreagentqualityofpotassium
chloride(KCl)andtransferitintothesamevolumetricflaskthroughthesame
funnel.
2)Adddoubledistilledwatertotheflask,dissolvedthecrystalsandmakeup
thesolutiontothemarkwithdoubledistilledwater.Thestockstandard
solutioncontains1000ppm/1000ppmofsodiumandpotassium.Fromthis
stockstandardsolution100,80,60,40,20ppmsolutionoflower
concentrationwasprepared.
Method
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6. 3)Aspirate Distilled Water and set the read out 00 by adjusting the zero control.
Aspirate the standard solution that has higher concentration adjust the nob to 100.
4)For optimum performance the instrument should be allow 15 min to warm up
during this warm up period a blank water sample should be aspirated. Emissions
were noted for all standard solution. Lastly water sample solutions were aspirated
and emission was noted.
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7. Characteristic Wavelengths Of The Elements
Element Emission wavelength (nm) Flame color
Potassium (K) 766 Violet
Lithium (Li) 670 Red (Carmine)
Calcium (Ca) 622 Orange
Sodium (Na) 589 Yellow
Barium (Ba) 515 Lime Green
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8. Procedure
1.Ensurethatthecompressorunit,LPGpipeareproperlyconnectedandsecured.
4.OpentheregulatorvalvefromLPGcylinder.
2.SwitchONthemainunitandcompressorunit.
3.Adjusttheairregulatorknobtoachieveareadingbetween0.4-0.6.
5.OpentheFuelcontrolvalvefrommainunit.
6.Inserttheigniterontheburnerandignitetheflame.Iftheflameisnotignitedwithinfewattempt
increasethefuelflowgraduallyoncetheflameisigniteditwillbeyellownon-oxidizingflame.Slowly
reducetheflamesoastogetablueoxidizingflame.Allowtheflametostabilizefor5min.
7.Takedistilledwaterinthebeakerandinsertthecapillaryaspiratorinserttherequiredfilter.
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9. 8.Adjustthezerocontrolto‘00’ondisplay.Removethedistilledwaterandplacethebeakercontaining
therepairedsampleofknownconcentrationandselectappropriatefilter.
9.Allowtheflametostabilizefor1-2min.
10.Adjustthecalibrationknobtoadjustthevalueofthestandardondisplay.
11.Repeattheoperationasgivenin8&10above.
12.Onpressingfreezeswitchthereadingwillbeaveragedandstored.
13.Remembertoaspiratewithdistilledwaterbetweentworeadingstoflushoutearliercontent.
14.Attheendoftheexperimentshut‘OFF’thefuelvalvefirstwhentheflameisextinguishedclosethe
airvalveandshutdownthemainunitandcompressorunit.
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10. •Theconcentrationofsodiumandpotassiumpresentinwatersamplecollected
fromriverwascompareagainststandardsolution(concentrationinppm)of
sodiumandpotassium.
•GraphwasplottedonYaxis(Intensityofemission)versesX-axis
(concentrationinppm)showsin(Figure1and2)
•TheconcentrationofsodiumandpotassiumionspresentinGodavariandDarna
(SampleA&B)riveris26ppmand08ppmand58ppmand14ppm
respectively.
•Concentrationofsodiumpresentinthewatersampleismorethanpotassium.
Result and Discussion:
10

11. Sr No.Concentration in ppm Emission for Na
+
Emission for K
+
1 20 24 26
2 40 36 42
3 60 58 61
4 80 78 80
5 100 100 100
6 Sample-A 58 14
7 Sample-B 26 08
Table-Emission for Na
+
and K
+
with respective concentration and
unknown sample.
11

12. Figure 1. Concentration of Sodium ions Figure 2. Concentration of Potassium ions
12

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Conclusion:
•Theconservationofriverisintheinterestofmanasit’secological,culturaland
touristvalueisimmense.
•Thisstudywillhelpinunderstandingtheamountofelementbeingreceivedin
theriveranditsbiologicalmagnificationinanimals,particularlythoseatthe
lowerleveloffoodchain.
•Thisstudywillalsohelptomakeawarethoselocalpeopleoradjacentfarmers
forpropermanagementofwastedisposalandalsotominimizeuseofsynthetic
inputs.
•Thestudyindicatedthatincreaseintoxicwastedaybydayinriverproduced
biologicalmagnificationinfoodchain,whichisachallengetoscientists,policy
makers,administratorsandallthoseinvolvedintheconservationofthe
environment.

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References
•ChikhaleHU,ChikhalePU(2017)Flamephotometricestimationofsodiumandpotassiumion
presentinwatersampleofDarnaandGodavariRiver.IntJSciEngRes8(1):131–136
•BWBTechnologiesUKLtd.AguidetoFlamePhotometerAnalysis.
•BiefeldP.F.et.al.(1958).DeterminationofSodium,Potassium,MagnesiumAndIronOxidesIn
PortlandCementByFlamePhotometry,ResearchLaboratoryOfficeofTestingandResearch
ReportNo.284.1-4.
•WorthH.G.(1985).Acomparisonofthemeasurementofsodiumandpotassiumbyflame
photometryandion-selectiveelectrode".Ann.Clin.Biochem.22:343-50.
•IndianStandard(1980)“Methodfordeterminationofsodiumandpotassium(FlamePhotometric)”
1-8.
•http://tera.chem.ut.ee/~koit/arstpr/nak_en.pdf(2012)
•BhorM.et.al.(2013).WaterQualityAssessmentoftheRiverGodavari,AtRamkund,Nashik,
(Maharashtra),India.InternationalJournalofEngineeringandScience2(2).64-68.

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