Fluorescent Cell Membrane Stains for Cell Imaging

The cell membrane defines the boundary between a cell's interior and its surroundings, playing key roles in signal transmission and molecular transport. We offer five membrane-selective fluorescent dyes that enable clear, consistent staining with minimal effort; simply add the reagent to your culture media. Each dye emits in a unique wavelength range, allowing for multiplexing with other fluorescent probes.

1. Fluorescent Cell Membrane Stains

2. Introduction The cell membrane defines the boundary between a cell's interior and its surroundings, playing key roles in signal transmission and molecular transport. We offer five membrane-selective fluorescent dyes that enable clear, consistent staining with minimal effort; simply add the reagent to your culture media. Each dye emits in a unique wavelength range, allowing for multiplexing with other fluorescent probes.

3. Products Product Number Product Name D6202 DiI Solution [for Cell Membrane Staining] D6263 DiD Solution [for Cell Membrane Staining] D6539 DiO Solution [for Cell Membrane Staining] D6540 DiR Solution [for Cell Membrane Staining] P3316 PKH 26 Solution [for Cell Membrane Staining]

4. Maximum Excitation/Emission Wavelength of Fluorescent Stains

5. Example of Membrane Staining with DiI Solution (Product No. D6202) Culture cells and remove medium. Add medium supplemented with  D6202  to a final concentration of 5 μM (staining medium), and incubate cells at 37°C for 10 minutes. Remove staining medium and wash three times with PBS(-). Observe cells via a fluorescence microscope. Figure: HeLa cells stained with  D6202

6. Example of Membrane Staining with PKH 26 Solution (Product No. P3316) Culture cells and remove medium. Add medium supplemented with  P3316  to a final concentration of 2 μM , and incubate cells at room temperature for 5 minutes. Add a volume of fetal bovine serum (FBS) equivalent to the volume of medium used in step 2, and incubate cells at room temperature for 1 minute. Remove medium with FBS and wash three times with PBS(-). Observe cells via a fluorescence microscope . Figure: HeLa cells stained with  P3316

7. Related Products Product Number Product Name C4078 DiR D6413 3,3'-Dioctadecyloxacarbocyanine Perchlorate D6451 DiD D6452 DiI M3842 Membrane Protein Extraction Kit P3233 PKH 26

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