Fluorimetry

Here we get most all things about fluorimetry used in pharmaceutical drug analysis

1. INTRODUCTION

2. Animation of principle of Fluorescence and Phosphorescence https://www.youtube.com/watch?v=AWkT9teuBlQ

3. EXAMPLE Fluorescent paint used on road sign boards

4. Example Radium used in watches shows phosphorescence

5. EXCITATION

6. HOMO & LUMO HOMO - Highest occupied molecular orbital LUMO - Lowest unoccupied molecular orbital

7. PATH LENGTH

8. QUENCHING

9. INSTRUMENTATION

10. MERCURY ARC LAMP

11. TUNGSTEN LAMP

12. FILTERS There are two filters for the fluorometer: The primary filter or excitation filter or incident light filter isolates the wavelength that will cause the compound to fluoresce (the incident light). The secondary filter isolates the desired emitted light ( fluorescent light ).

13. M0N0CHROMATORS A monochromator is an optical device that transmits a mechanically selectable narrow band of wavelengths of light or other radiation chosen from a wider range of wavelengths available at the input. Greek words mono- , "single", and chroma , "colour"

14. SAMPLE HOLDER / CUVETTE

15. PHOTOMULTIPLER TUBE

16. Animation of instrumentation of Fluorimetry https://www.youtube.com/watch?v=zGFQEbdeAF0

17. SINGLE & DOUBLE BEAM FLUORIMETER

18. APPLICATIONS OF FLUORIMETRY

19. TAPAN KUMAR MAHATO [email protected]