General principle and types of chromatography

A basic understanding of chromatography, various types and how it works

1. General Principle and types of chromatography By, Ritwick Ranjan Sarma M.Sc. Bioscience Yenepoya Research Centre

2. Contents: History Introduction Principle Chromatogram Retention Factor Types of chromatography TLC Gas chromatography HPLC Size exclusion chromatography Affinity chromatography Ion exchange chromatography Applications of chromatography

3. History: Tswet , a Russian Botanist (referred to as the father of Chromatography) is credited with the development of chromatography in the year 1903. Used this technique to separate plant pigments. http://insertmedia.office.microsoft.com

4. Chromatography: The word “Chromatography” is derived from two greek words- Chroma means colour, and Graphein to write . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. http://insertmedia.office.microsoft.com

5. Principle of Chromatography: Chromatography is based on the principle of the partition of solute between two phases/solvents. Consist of mobile phase and stationary phase. http://insertmedia.office.microsoft.com

6. Chromatogram: A graphical representation of detector response, concentration of analyte in effluent, or other quantity used as a measure of effluent concentration. The retention time or volume is when a solute exists the injector and passes through the column and the detector. http://insertmedia.office.microsoft.com

7. Retention factor: http://insertmedia.office.microsoft.com

8. Types of Chromatography:

9. Thin Layer Chromatography(TLC) http://insertmedia.office.microsoft.com

10. Gas Chromatography: http://insertmedia.office.microsoft.com

11. HPLC: http://insertmedia.office.microsoft.com

12. Size Exclusion Chromatography http://insertmedia.office.microsoft.com

13. Affinity Chromatography: http://insertmedia.office.microsoft.com

14. Ion-exchange Chromatography: There are two types of Ion exchange Cation exchange Anion exchange Column is made up of polymer which is attached to a charged ion ( cation /anion). Stationary phase = Solid Mobile phase = Liquid

15. Application of chromatography: http://insertmedia.office.microsoft.com

16. References: Wilson and Walker Biochemistry, 6 th edition. https://microbenotes.com/chromatography-principle-types-and-applications https://www.omicsonline.org/special-issue/chromatography-and-its-applications.php www.slideshare.net/chromatography