Growth Performance of Common Duckweeds in Aquashade Tech using different types of fertilizers

Duckweeds culture under aquashade net

1. FIELD PRACTICE AT THE PROVINCIAL INSTITUTE OF FISHERIES ISABELA STATE UNIVERSITY ROXAS CAMPUS, ROXAS, ISABELA WITH EMPHASIS ON THE COMPARATIVE ANALYSIS ON THE EFFECTS OF FERTILIZERS UNDER UNSHADED AND AQUASHADE TECHNOLOGY ON THE GROWTH PERFORMANCE OF COMMON DUCKWEED Lemna minor Presented by: Rosalinda V. Ole Researcher Mary Joy L. Asprec, PhD. Adviser 1

2. Internal Assessment External Assessment STRENGTHS WEAKNESSES Availability of area Excellent faculty and staff Good water system Limited materials for the conduct of experiments Lack of technical equipment OPPORTUNITIES SO STRATEGIES WO STRATEGIES Increasing of laboratory equipment Popularity of the agency Expansion of production area Sustain additional modern and functional facilities Improve the area Purchase new functional equipments for experiments THREATS ST STRATEGIES WT STRATEGIES Limited literacy in using laboratory equipments Poachers Level up and maintain the security of the area Proper management and monitoring of the site and its resources Strength-Weaknesses-Opportunities-Threats (SWOT) ANALYSIS of the Provincial Institute of Fisheries 2

3. COMPARATIVE ANALYSIS ON THE EFFECTS OF FERTILIZERS UNDER UNSHADED AND AQUASHADE TECHNOLOGY ON THE GROWTH PERFORMANCE OF COMMON DUCKWEED Lemna minor 3

4. 4 INTRODUCTION Global Warming Problems in Production of Crops Fossil Fuels Usage Threatens Food and Feeds Supply How to ameliorate these problems?

5. INTRODUCTION Duckweeds are among the smallest free-floating aquatic plants worldwide. Duckweeds are aquatic plants belonging to the botanical family Lemnaceae . The family consists of five genera, Lemna, Spirodela , Landoltia , Wolffia , and Wolffilealla (Les et al., 2002). Potential feedstock for bioethanol production because of its high levels of starch accumulation ( Xu et al., 2011; Xiao et al., 2013). Proteins Carbohydrates Fats Contain a variety of secondary metabolites that are beneficial to humans ( Baek et al., 2021). 5

6. 6 OBJECTIVES 1 3 2 4 To determine the difference of growth performance of common duckweeds Lemna minor , supplemented with chicken manure and commercial fertilizer To determine the difference of growth performance of common duckweeds Lemna minor under unshaded tanks and aquashade technology; To determine if there is an effect of using shading and non-shading method, with the supplementation of different fertilizers, in the weight gain of the common duckweeds To determine the effects of water temperature and weather temperature in the growth of duckweeds

7. 7 COMMON DUCKWEEDS, Lemna minor Taxonomical Classification of Common Duckweed, Lemna minor Kingdom: Plantae Phylum: Tracheophyta Class: Liliopsida Order: Alismatales Family: Lemnaceae Genus: Lemna Species: minor Scientific Name: Lemna minor Common Name: Common Duckweeds Common Duckweed, Lemna minor

8. 8 COMMON DUCKWEEDS, Lemna minor Biology of Common Duckweeds MORPHOLOGY Lemna minor. Three-frond colony (a)top view; (b) side view ( Hasan and Chakrabarti , 2009 )

9. 9 COMMON DUCKWEEDS, Lemna minor Biology of Common Duckweeds ECOLOGY Duckweed species are adapted to a wide variety of geographic and climatic zones, with most species inhabiting tropical and subtropical areas ( Mwale and Gwaze , 2013 ). They do not grow in waterless deserts and permanently frozen areas.  Lemna  species are very rare in regions with high or very low precipitation ( Landolt and Kandeler , 1987 ) . Duckweeds usually float on or just below the surface of nutrient-rich still or slow moving bodies of fresh and slightly brackish waters.

10. Legends: Biology of Common Duckweeds REPRODUCTION COMMON DUCKWEEDS, Lemna minor 10 M Mother Frond D Daughter Frond

11. 11 METHODOLOGY EXPERIMENTAL SET-UP A pproximately 20-30 cm Fertilizers Water depth Chicken Manure 16-20-0 Aquashade Technology Traps sunlight approximately 90%

12. 12 METHODOLOGY Collection of duckweeds Preparing of the culture tanks Water filling Preparing the fertilizers Stocking of duckweeds in culture tanks Installation of aquashade net Application of fertilizer Sampling OPERATIONAL PROCEDURE

13. 13 METHODOLOGY Statistical Analysis Two-way ANOVA With replication Correlation t -Test T o compare the difference in growth performance of common duckweed under shaded and unshaded culture tanks which are supplemented with organic fertilizer and inorganic fertilizer. To assess the effect of aquashade technology and the use of different fertilizers in the weight gain of common duckweeds To assess the relationship between the water temperatures, weather temperature, and the WG of common duckweeds. 13

14. 14 RESULTS AND DISCUSSION Effects of types of fertilizers on growth performance of common duckweeds, Lemna minor Table 4. t -Test on growth performance of duckweeds under shaded tanks supplemented with organic manure and commercial fertilizer. Table 5. t -Test on growth performance of duckweeds under unshaded tanks supplemented with organic manure and commercial fertilizer.

15. 15 RESULTS AND DISCUSSION CYCLE ORGANIC INORGANIC 1 SHADED 424 545 2 431 558 3 429 552 4 438 549 5 435 540 1 UNSHADED 303 793 2 314 815 3 326 765 4 310 748 5 319 751 Table 3 . Weight gain of common duckweeds cultured in shaded and unshaded outdoor tanks supplemented with organic and commercial fertilizer (in grams g ).

16. 16 RESULTS AND DISCUSSION Effects of unshaded and aquashade technology on growth performance of common duckweeds Lemna minor. Table 6. t -Test on growth performance of duckweeds under shaded and unshaded tank supplemented with organic manure. Table 7. t -Test on growth performance of duckweeds under shaded and unshaded tank supplemented with inorganic manure.

17. 17 RESULTS AND DISCUSSION CYCLE ORGANIC INORGANIC 1 SHADED 424 545 2 431 558 3 429 552 4 438 549 5 435 540 1 UNSHADED 303 793 2 314 815 3 326 765 4 310 748 5 319 751 Table 3 . Weight gain of common duckweeds cultured in shaded and unshaded outdoor tanks supplemented with organic and commercial fertilizer (in grams g ).

18. 18 RESULTS AND DISCUSSION Aquashade technology and different fertilizers in common duckweed growth performance. Table 8. ANOVA on the effects of type of fertilizer and aquashade technology on growth performance of common duckweeds

19. 19 RESULTS AND DISCUSSION Effect of weather and water temperature on the growth performance of common duckweeds. Table 9. Correlation on the growth performance of common duckweeds and water temperature and weather temperature.

20. 20 CONCLUSION In terms of the effect of different types of fertilizers as a main factor, inorganic fertilizer has a much better effect in growth performance of common duckweeds than organic fertilizer. With regards to the effect of shades, unshading and aquashade technology, as a main factor in the growth performance of common duckweeds, aquashade technology has positive effect on the organic supplementation while unshading is much better in inorganic supplementation. However, other factors should be taken in consideration, such as acidity of the water and the light intensity. 1 2

21. 21 CONCLUSION With regards to the relationship between water temperature, weather temperature, and growth of common duckweeds, the growth of common duckweeds was not dependable to the water and weather temperature, the two parameters have no significant relationship to the growth of common duckweeds 3 4 In terms of the effect of different types of fertilizers and shading method, when these factors are combined, growth of common duckweeds can fluctuates depending on the other factors that may affect the growth of the plant.

22. 22 Thank you!