Hotis test

test for mastitis
used in veterinary field

1. HOTIS TEST ABHIJITH SP CVAS POOKODE

2. INTRODUCTION This test has recently been introduced, and is supposed to be of a useful diagnostic procedure. Used to detect mastitis. Discovered in 1943. A test for the presence S. agalactiae in bovine mastitis in milk. This test is now also used and extended to find mastitis producing Streptococci. 14-09-2020 ABHIJITH SP CVAS POOKODE 2

3. PROCEDURE In this test 9.5 ml of milk is taken in a test tube, add 0.5 ml of bromocresol purple. Tube is inverted several times. Incubate at 37 degree centigrade for 24 h. If positive , canary yellow colonies will be found on the sides of test tube. When the dye and milk are first mixed, the color is purple. If streptococci are present the color changes during incubation from purple to green or yellow as a result of acid production. If the Streptococcus agalactiae is present small flakes or balls of growth canary yellow in color became deposited on the side of the tube. 14-09-2020 ABHIJITH SP CVAS POOKODE 3

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