HPLC In Pharmaceutical sciences

High performance liquid chromatography, Uses of HPLC in pharmaceutical science

1. Jagannath University, Dhaka
Department of Pharmacy
Assignment On
“Uses of HPLC In Pharmaceutical Sciences”

Course Code: PHAT-3101
Submitted To:
Dr. Mohammad Musarraf
Hussain
Associate Professor
Department of Pharmacy
Jagannath University
Submitted By:
Boni Eiamine Rafi
ID: B170606022

Department of Pharmacy
Jagannath University

Date of Submission 10
th
sept 2021

2. INTRODUCTION
Chromatography is a laboratory technique for the separation of a
mixture. The mixture is dissolved in a fluid called the mobile phase,
which carries it through a system on which a material called the
stationary phase is fixed.
High-performance liquid chromatography, formerly referred to as
high-pressure liquid chromatography, is a technique in analytical
chemistry used to separate, identify, and quantify each component in a
mixture.
High performance liquid chromatography (HPLC) has been widely used
for years as an analytical method and is a key tool for the separation and
analysis of pharmaceutical drugs, for drug monitoring and for quality
assurance and life science research. HPLC is commonly used for
Separation and analysis of non-volatile compounds. Due to the flexibility
of this technique numerous applications have been adopted for routine
use in the pharmaceutical industries, research laboratories, analytical
laboratories, clinical laboratories and also colleges.

Liquid chromatography is the science of separating the chemical
compounds that are in the sample. Afterward, these chemical
compounds can identify and quantitate what is. Reversed-phase HPLC
(RP-HPLC) is one of the more popular methods due to its speed, column
stability, and capacity to separate a wide range of compounds. There are
two variants of HPLC, depending upon the phase system (stationary) in
the process. This method separates analytes on the basis of polarity.
NPHPLC uses polar stationary phase and non-polar mobile phase.
Therefore, the stationary phase is usually silica and typical mobile phases
are hexane, methylene chloride, chloroform, diethyl ether, and mixtures

3. of these. Polar samples are thus retained on the polar surface of the
column packing longer than less polar materials. In case of Reverse Phase
HPLC the stationary phase is nonpolar (hydrophobic) in nature, while the
mobile phase is a polar liquid, such as mixtures of water, methanol,
acetonitrile (or) mixtures of these. It works on the principle of
hydrophobic interactions hence the more nonpolar the material is, the
longer it will be retained. Reversed-phase HPLC is by far the most popular
mode chromatography. Almost 90% of all analyses of low molecular
weight samples are carried out using RP- HPLC.

Uses of HPLC in pharmaceutical science
HPLC industry Applications: There is a wide variety of applications
throughout the process of creating a new drug from drug discovery to
the manufacture of formulated products that will be administered to
patients. This Process to create a new drug can be divided into 3 main
stages 1. Drug discovery 2. Drug development 3. Drug manufacturing.
LC-MS is the best tool for compound identification and characterization.
It may be used as a measurement tool during high throughput screening.
Preparative HPLC is also used to isolate and purify hits and lead
compounds as required. Eg: a combinatorial synthesis. The ability to
prove purity of enantiomeric molecules is a standard in pharmaceutical
assays, for which HPLC is suitable.
Pharmaceutical applications:
1. Tablet dissolution study of the pharmaceutical dosage form.
2. To control drug stability, Shelf-life determination.
3. Identification of active ingredients.
4. Pharmaceutical quality control.

4. Pharmaceutical impurity profiling analysis:
1. Structure elucidation of impurities with LC/MS.
2. Rapid condition scouting for method development.
3. Using a fast LC method for higher sample throughput
Pharmaceutical drug discovery analysis:
Developing a fast, generic method for rapid resolution Liquid
chromatography with quadrupole MS detection. Fast, generic LC/MS
method enables drug analysis in less than one minute.
Recent applications in pharmaceutical science:
Analytic method development and validation are key elements of any
pharmaceutical development program. HPLC analysis method is
developed to identify, quantity or purifying compounds of interest. HPLC
helps a lot in stability studies of drug formulations. HPLC helps a lot in
stability studies of atropine, antibiotics, & biotechnology-based drugs
like insulin, streptokinase, etc.
1. It is used for separation and identification of Psychotropic drugs such
as antidepressants, benzodiazepines, butyrophenones, neuroleptics,
phenothiazines, etc.
2. It can be used for determining the stability of various pharmaceuticals.
This is done by analyzing the degradation products of the drugs Eg:
Stability studies of atropine
3. It can be used in bioassays of compounds like chloramphenicol,
cotrimoxazole, penicillin, peptide hormones, and sulfonamides.
4. It is used for controlling microbiological processes used in the
production of the number of antibiotics such as chloramphenicol,
tetracyclines, and streptomycin.

5. 5. It is used for monitoring the course of organic synthesis and also for
isolating products in the reaction.
6. It is used for monitoring the course of organic synthesis and also for
isolating products in the reaction.
7. It gives an idea about the biopharmaceutical properties of a dosage
form and the pharmacokinetics of the drugs. Thus, it is used in dosage
form design.
8. It is utilized as an analytical method for numerous natural and
synthetic drugs. It is used in different levels of pharmacy and
pharmacology.

CONCLUSION
High-performance liquid chromatography is just the premier technique
for Trace analysis of organic and inorganic compounds. Determination of
trace compounds is very important in pharmaceutical, biological,
toxicology and environmental studies since even a trace substance can
be harmful or poisonous. HPLC is applied for molecular weight
determination, in analytical chemistry, pharmaceutical and drug science,
clinical sciences, food technology, and consumer products,
combinatorial chemistry, polymer chemistry, environmental chemistry.
The role of HPLC in the pharmaceutical industry is very vital particularly
in pre-formulation, process development, during formulation
development and drug discovery and to verify drug purity. HPLC is the
best separation technique for quantitative trace analysis of toxic
chemicals, impurities, Manufacturing of high pure products, medicinal
uses, and research purpose.

6. REFERENCES
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