IB DNA Structure & Replication Review Key (2.6-2.7-7.1)

Review worksheet answer key covering IB Biology content in DNA structure and DNA replication (Topics 2.6, 2.7, and 7.1)

1. 18 ilogy Ht: DNA Suche à Reptcoton Review se Koy
1. Due ag met eng ete dene

sur and nitrogen basen the box tothe ih: Prost Aen
scteeiie: Deoxyribose Seeger

2. Compare DNA and RNA.
DNA

o o RNA
Similares Kuelee acid polyners made up of necleotides
+ Contain moyen uses Adenine, Cyfesine, and Crumine
Dre

Deoxyribose sogar Bibose Sugar
Devkle-strundel (hebix) Single-stianded (om-helix) |
Thupaive 15 hole nitrogen buse | Uracil 35 fourth bise.

segment of DNA with four nucleotides (arranged in two base pairs}

Nite
pas]

A. Tre agram shows DNA double eli How does a double hei pr? What bonds hol in postion?
DNA devble helix is formed by hos elote Sands |
fearing autipara one duother. Covalent bonds join nucliok
in Seyor phosphate beck bene. Hydrogen fonds join pibregen haze pairs,

AL
mo EE Ju
vile

6. What are the funtion of nucleosomes?
Necleosonres alle packaging /supereoiting of DIVA Ile chromosomes Lor ex
paar aprte (melas, When PNA is’ ue ier es it canot

ranseribed/praesiakad so huis albus genes to "otf."

2, ne Sa Meee i ot secs

Nude séquences in DIA that do not code for production of proteins.
also albA ter, Refenting ce leer patterns are "5-30 laters Ion

fhe. E
aud build. op mutations, quickly without alfectin Spa (inc thay dont de or nat
cala ral Ham apt ee tsa Fergie, hey also er] telomeres ,
Produce {POA and TEVA, and hulp promote silence gone expression.

2. 8 Waitomaten i ost rm out sect
+X patter showed DNA was a helix + Ditange From couter te Sop she.
+ Ange X showedd heupness of helix mie cf one: lal ben (10 as
+ Distance behusen bors = dichwce beweon bases (tum)

9. “cutie how the Hershey-Chase experiment showed DNA, aot roten, was the genetic mater
Gina À wae Poux Wat vireses infected cells by Injecting, anche material inte
tre coll, May labeled the protein in ona ge of TZ Pustensphigen amd the DNA in
the olla (fin grup had rdirache sohhr— Dit gp bd radoative phesphere), Whee he
prodein-labelel vinses infected Ha cells, there cons ne Padioactviby olzserail in ble cells
Wien the DIA lb viruses infected cells, there cons dbiactivity in tha e

in sl ae eas a e ds puts EBEN,

To creake nas, dential coples of DMA molecules noel lr when cells divide.

11, Explain the meaningof OWA repictions semiconeratie: | a ANSE
Each nen DNA molacale, contains one old" strand From Ihe original mdec
and on Mal - spathasiccd strand. Maschen and Stobl porel U in Ha hire
2, nuts mini gr carte 2

Shuchre and number of hydoyen bonds mud between buses LA)
make ih Moby unlikely Hat the bases will Le incmechy potred dering replication,
13. Describe how the Polymerase Cain Reaction (PCR) works, indi the rol of Tag DNA Polymerase: .
PCR ostificially recreates DU replicabion process ls ampldy small Samples of PUA.
DPRAsaple is haskell le 95°C ty separado shrunls
ee nee ace col to AE and primers ach te tagt DOA sequence.
Pestle Tey Du se alle neo nyelatihes to copy ha uh sepa
nn apie ee tale oer omer fee
14 Complete the table with the functions ofthese Me enzymes involve in DMA replication.

Enzyme Function
_ [Omaña DAM Yin and Dred hugo beds te separate shanks |
NA Primase Hals RNA radactides fo Lona primers on Hu Lajginy hou of DAY.
omaPohmerssei [Ads DA zuelzchdes in 73° Urection of hth Shands,

onavohmerase! [faces RUA prints on lagging Share with DIA reclestibes
Coponomerae) [bee Ve hall a9 tb i uneound + vntelppad by Helicase

Fopotomerse)
NA pase dns tsgelhor Oecd! Frugments on lagging rand.

15. What are Okczak fragments?

Fragments of DNA copied discomincoy on ae lagi) Shan during plication

Ain ran does DIA repo decir a. DE ie
ps = CEA sopence
17. Describe the use of Dideosyribnonucleotides to sequence unknown DNA samples, 77 Eee

Flvorescentig -labled dANTPs are added into mix of vrknown DNA sequence, normal mule
a ae Gee Repliaton cer ont a JUNTO ge abcd and cops the Seow) at
aspecitic hase läher (hich cun be dehermined by Mu cole! label of the bso), The Pagments are
Separated by size, te determine the. (ater sequbece by leek ing al smallest lo largest hagments