IMMUNOFLORESCENCE ED. Microbiology pptx ...........

Microbiology

1. IMMUNOFLUORESCENCE Sudharsanam k MVM230 45 ( vpe )

2. history Sir George G. Stokes (1852), made the first observation that the mineral 'fluorspar' exhibits fluorescence when illuminated with ultraviolet light, and he coined the word "fluorescence"

3. introduction Technique allowing the visualization of a specific protein or antigen in tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye

4. Principle of fluorescence • Property of certain molecules or fluorophores to absorb light at one wave length (excitation wavelength) and emit light at different wave length (emission wavelength) • Selective filters used for this purpose

5. Diagnosis Of Animal Rabies By Immunofluorescence

6. DIRECT INDIRECT TIME Shorter procedure, single labelling Longer , double labelling step COST Expensive less COMPLEXITY Less complex more FLEXIBILITY limited greater SENSITIVITY less High, amplification of signal DIRECT VS INDIRECT IF

7. (paraformaldehyde)