InsilicoPCR.pptx

Overview of InsilicoPCR an alternative to conventional wet lab pcr tool

1. Exploring the Virtual Laboratory: In Silico PCR ~ Pranavi Uppuluri 1

2. Here We Consider WHAT IS PCR? TYPES OF PCR? COMPONENTS OF PCR STEPS IN PCR REACTION CYCLE WHAT IS INSILICO PCR? WHY IS INSILICO PCR? PROTOCOL FOR INSILICO PCR INSILICO PCR TOOLS APPLICATIONS OF PCR Pranavi Uppuluri 2

3. Pranavi Uppuluri 3 WHAT IS PCR Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail. PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified, and then multiple rounds of DNA synthesis to amplify that segment.

4. Kary Banks Mullis (December 28, 1944 – August 7, 2019) was an American biochemist. In recognition of his role in the invention of the polymerase chain reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith KNOW THE INVENTOR 4 Pranavi Uppuluri

5. 5 TYPES OF PCR Pranavi Uppuluri

6. Pranavi Uppuluri 6 COMPONENTS OF PCR

7. Pranavi Uppuluri 7 Steps INVOLVED IN PCR

8. Pranavi Uppuluri 8 REACTION CYCLE

9. WHAT IS INSILICO PCR In silico PCR is a computational method used to simulate and analyze Polymerase Chain Reaction (PCR) reactions using computer software and algorithms rather than physically conducting PCR experiments in a laboratory. It allows researchers to predict the outcomes of PCR reactions, including the expected amplicon size, location on the target DNA, and the specificity of the primers, all through computational analysis. In silico PCR is a valuable tool for experimental planning, primer design, and verifying the feasibility of PCR experiments without the need for physical laboratory work. Pranavi Uppuluri 9

10. Pranavi Uppuluri 10 WHY IS INSILICO PCR

11. Pranavi Uppuluri 11 PROTOCOL FOR INSILICO PCR

12. Pranavi Uppuluri 12 INSILICO PCR TOOLS Performing in silico PCR can be done using various bioinformatics tools and software that are specifically designed for this purpose .

13. Pranavi Uppuluri 13 APPLICATIONS OF PCR

14. Pranavi Uppuluri 14