Ion chromatography

PRINCIPLE, INSTRUMENTATION, WORKING PROCEDURE AND APPLICATIONS OF ION EXCHANGE CHROMATOGRAPHY

1. ION CHROMATOGRAPHY PRESENTED BY : MR K V NANDA KUMAR ASSOCIATE PROFESSOR DEPARTMENT OF PHARMACEUTICAL ANALYSIS KRISHNA TEJA PHARMACY COLLEGE, TIRUPATHI - 517506 1

2. CONTENTS 27-05-2021 B PHARM PA II - MR K V NANDA KUMAR 2

3. 27-05-2021 B PHARM PA II - MR K V NANDA KUMAR 3

4. INTRODUCTION B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 4

5. TIMELINE OF ION CHROMATOGRAPHY B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 5

6. TYPES OF ION CHROMATOGRAPHY B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 6

7. B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 7

8. THEORY OF ION CHROMATOGRAPHY B PHARM PA II - MR K V NANDA KUMAR 5/27/2021 8

9. IONIC EXCHANGERS B PHARM PA II - MR K V NANDA KUMAR 5/27/2021 9 Anionic exchangers have positively charged groups that will attract negatively charged anions. These are also called “Basic ion exchange” materials. Cationic exchangers possess negatively charged groups, and these will attract positively charged cations. These exchangers are also called “Acidic ion exchange” materials because their negative charges result from the ionization of acidic groups.

10. TYPES OF ION EXCHANGE RESINS B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 10

11. PREPARATION OF THE EXCHANGE MEDIUM B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 11

12. ION CHROMATOGRAPHY PROCESS The basic process of chromatography using ion exchange can be represented in 5 steps (assuming a sample contains two analytes A & B) : eluent loading, sample injection, separation of sample, elution of analyte A, and elution of analyte B , shown and explained below. Elution is the process where the compound of interest is moved through the column. This happens because the eluent, the solution used as the solvent in chromatography, is constantly pumped through the column. The representative schemes below are for an anion exchange process. Eluent ion = Ion A= Ion B = B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 12

13. STEP 1 B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 13

14. STEP 2 B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 14

15. STEP 3 B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 15

16. STEP 4 B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 16

17. STEP 5 B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 17

18. INSTRUMENTATION Typical IC instrumentation includes: pump injector column suppressor detector and recorder or data system B PHARM PA II - MR K V NANDA KUMAR 5/27/2021 18

19. WORKING B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 19

20. APPLICATIONS OF ION EXCHANGE CHROMATOGRAPHY B PHARM PA II - MR K V NANDA KUMAR 27-05-2021 20

21. THANK YOU 5/27/2021 B PHARM PA II - MR K V NANDA KUMAR 21