Ion Exchange chromatography by Mr. Vinayak

Introduction
Classification of Resins
Factor affecting ion exchange
Methodology
Applications

1. Ion Exchange Chromatography

So A s
= —

won’
A Vinayak R. Bodhankar_ de

4 M. Pharm. (Ph.D*) =

3. $ INTRODUCTION

4. < PRINCIPLE
e: principle of separation is by reversible exchange of ions between theft

ent in theisample solution to the ions present on ion exch:

> R s the from sample and release ion from-itse

attract negatr

> materials.

5. ar

1% A Ye eas # — -

U 2 N lon exchange
a Chromatography

Stationary
phase + + à
RAR A Sample
(Analyte)
/ (HE) (Se So) NX
lon exchange
, Cation Exchanger
| Chromatography | / (Have negative charge)
Stationary | ;

y Anion Exchanger

phase /
| F (Have positive charge)

6. « CLASSIFICATION OF ION EXCHANGE RESINS : :

the basis of Chemical nature

7. _ UO Ideal properties of Resins
t should:be Cheap, Easily available

8. 10-port
y | switching
valve

CAS
Anion- he S
exchange y || N y ) y
Eluent column N AL
JAN Injection

Drain value

Data
processor

t

Cation-
exchange
column

Conduetivity
ES detector
Sampling
syringe

9. PRACTICAL REQUIREMENTS / METHODOLOGY

Column material and dimension E 4
e of iomexchange resin & the selection depend:on following properties

10. 1. Column material and dimensions:

‘olumns'used in the laboratories are made up of glass. In industries are-ma

r high quality stainless steel or polymers which'aré |

11. Ton ‚exchange capacity:

is the. totabi jon Se capacity in terms of the ee functional

12. .:4. Mobile Phase:

rganic solvents are less useful and they are not used at all.

s of acids, alkalies and buffers are used as eluting so.

osphate buffer,

13. «

6. Analysis of the Effluent:

Several methods of analysis can be used which depends up on the nature &

14. + FACTORS AFFECTING ION EXCHANGE SEPARATIONS:

: ture and properties of ion exchange resins. os
ture) changing ions. ; =)

15. _ 2. Nature.of exchanging ions:

16. 5 MECHANISM OF ION EXCHANGE RESIN

17. th € 4 €

, + APPLICATIONS