IWFAS24_I Gusti Lanang Bumi Agung_SLR_Barr Body.pptx

best oral presentation

1. Sex Determining Based On Presence of Barr Body on Human Cell (a Systematic Literature Review) I Gusti Lanang Bumi Agung 1 1 Resident of Forensic Medicine and Medicolegal, Universitas Airlangga , Indonesia https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia INTERNATIONAL WORKSHOP AND THE 1 st FORENSIC ANTHROPOLOGY SYMPOSIUM 2024

2. PARIS 2024 Olympic The distinguise in determining of sex on major event. https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia Introduction

3. Barr Body is the small darkly stained structure, in the nucleus. It is also called as X / Sex Chromatin . Basically this deactivated or inactive X Chromosome. Introduction https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia Bhardwaj, N. (2022).

4. Introduction https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia The Process of Barr Body formation.

5. Problem Statement : Is determining of sex using Barr Body method can be applied at RSUD dr Soetomo ? Methodology https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia Objective : To know more about determining of sex using Barr Body method.

6. Methodology https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia Time of Research Research Methode Research Instrument Periode of Publication Research Gate, Pub Med, Google Scholar 10 years from 2013 - 2023 Jan-Mar 2024

7. https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia Keywords And Identification of Sex Barr Body Determination of Sex X Cromatin Methodology – Key Word OR

8. Methodology- Boolean Searching https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia

9. Methodology https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia Journal form Google Scholar (n=20 2 ) Journals from PubMed (n=180) Journal through screening phase (n=469) Journal that exclude (n= 364 ) Journals from Research Gate (n=87) Journal that eligible through next phase (n= 105 ) Journal seem complete but excluded due to the sample uses not from human, other due to the journal pulished more than 10 years (n=9 5 ) Journal that eligible through next phase that part of qualitative study (n= 5 ) Journal that eligible through next phase that part of quantitative study (n= 5 ) Data collection process

10. Results https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia

11. Results https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia P IC O P IC O

12. Results https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia P IC O

13. Results https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia Study Results Bharadwaj, (2022) The Barr Body sample count is bigger in female teeth sample than male sample. Datar, V (2013) Percentage barr body count on female buccal scraping sample bigger than male sample. Katakwar , V (2023) This study using two kind of staining sample on buccal mucosal swab; Metylen blue and Giemsa, which the result are 86,66% barr body positive on female sample that used metylen blue staining and 91.6% positif barr body on Giemsa staining. Laksmi, A (2023) Barr body positive on buccal mucosal swab. PAP staining male: 0%, female: 3-8%, transgender 0-5% and male: 0%, female: 1-3%, transgender: 0% on AO staining. Lashari, MH (2018) Barr body sample on blood smear count on female PMN cell is bigger than male PMN cell. Pathak, DB (2022) This study uses three type staining on buccal mucosal swab. CF staining 9,8% on female, 0% on male. MGG staining 5,4% on female, 0% on male. VG staining 2,8% on female, 0% on male. Ragunathan, YT (2023) This study uses three staining on teeth sample. Feulgen, AO and PAP staining show that barr body 100% on female teeth sample, and 15% on male teeth sample. Kulkarni, (2023) This study uses three staining on buccal mucosal swab sample. Which the result as below: Feulgen 30,2-37,9% on female, and 0-0,1% on male buccal swab sample. Giemsa 19,2-22,2% on female, and 0-0,3% on male buccal swab sample. Routine staining 10,2-14,9% on female, and 0-0,5% on male buccal swab sample. Deddy, AU (2017) This study uses teeth sample that burned in various degree celcius , which the result are: 100 C, 200 C, and 400 C: Barr body positive on female teeth sample and negative on male teeth sample. VDP, (2021) This study uses buccal smear as sample: AO staining and PAP staining show that barr body count is bigger in female sample than male sample.

14. Discussion https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia The Implication of this study : The research data indicates that Barr Body is consistently present in woman somatic cell  A New Alternative method to determine human sex. Can we do at Soetomo Hospital? Absolutely Yes  it’s quiet simple and more cost-effective compared to advanced methods such as DNA analysis.

15. Conclusion https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia THE IMPORTANCE ASPECT OF THIS STUDY: Due to economical aspect, the barr body should be a good choice than other advanced method. LIMITATION OF THE STUDY Despite barr body appearance is a sign that cell must be a woman, in contrast not all woman shown this sign (±20 %)  there is another method look like barr body that can be another alternative like Davidson body, etc. (need comparative study)

16. CONTACT https://fisip.conference.unair.ac.id/forensicanthropology ASEEC, UNAIR, Surabaya, Indonesia INTERNATIONAL WORKSHOP AND THE 1 st FORENSIC ANTHROPOLOGY SYMPOSIUM 2024 I GUSTI LANANG BUMI AGUNG UNIVERSITAS AIRLANGGA https://www.linkedin.com/in/bumi-agung-859613165/ https://www.instagram.com/bumiagunk/?hl=en 085935354301 [email protected]