Kozak sequence .pdf

Kozak sequence .pdf

1. Kozak sequence

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A consensus sequence (optimally CCA/GCCAUGG) located around the translation
start site and involved in its recognition by the ribosome.

Kozak sequence

Corresponding to the ribosome binding site (RBS) of prokaryotes, the sequence of
eukaryotic mRNA located on both sides of the initiation codon AUG is usually
ACCACCAUGG) by binding to the translation initiation factor to induce a 5-cap
structure. mRNA translation starts, this sequence is called Kozak sequence. Among
them, the first base G after AUG and the previous third base A or G are essential for
translation initiation; in addition, the 15 bases upstream of AUG generally do not
contain T [1].

Kozak sequence: Kozak consensus sequence, Kozak consensusor Kozak sequence, a
sequence that exists in eukaryotic mRNA and plays an important role in the initiation of
translation.

The ribosome can recognize this sequence on the mRNA and use it as the translation
start site. Note that this sequence is not a ribosomal binding site (RBS) ribosome
binding site, but a cap structure.

The best sequence upstream and downstream of the start codon in eukaryotic genes is
that the -3 position is a purine base; the +4 position is G, and the A in the start codon
AUG is in the +1 position.

A/GCCAUGG is called a kozak sequence or scanning sequence.

2. KOZAK is a female scientist. She has studied the effects of site-directed mutations
around the start codon AUG on transcription and translation, and concluded that in
eukaryotes, the sequence at both ends of the start codon is: = G/
N-CIN-C/N-ANNAUGG-, such as GCCACCAUGG, GCCAUGAUGG, has the
highest transcription and translation efficiency|Especially the A at position -3 is very
important for translation efficiency. This sequence is called Kozak sequence by later
generations and is applied The expression vector is under construction.