Lab Biosafety Day 1 presentation for workers.pptx

Lab Biosafety Day 1 for workers

1. Lab Biosafety – D1 Dr. Ei Ngon Phoo 1 Biosafety

2. Hazard Identification Biological Hazards Chemical Hazards Physical Hazards, Electrical Hazards Psychological Hazards Environmental Hazards Ergonomic Hazards Radioactive hazards Hazard is not the risk without a specific environment & situation. Biosafety 2

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13. Laboratory Staff responsibilities Know the biological materials and procedures used in the laboratory Follow approved lab procedures and safety guidelines Know the symptoms of infection with pathogens worked with in the laboratory Know emergency procedures Complete all required training before conducting any lab activity Utilize all requires Personal Protective Equipment (PPE) Use appropriate lab equipment and containment facilities Report any unsafe conditions to the biosafety officer or laboratory manager 13 Biosafety

14. General Emergency Information Emergency contact: Assistant Laboratory manager (Administration): [phone] Laboratory manager (Application): [phone] Biosafety Officer: [phone] First Aid Provider: [phone] Myanmar Fire Service Department: 191 Emergency power outage: [phone], [phone] Emergency Ambulance Service (MMA) 1830 14 Biosafety

15. Location of fire extinguishers: Biosafety 15 Ground Floor Second Floor At Reception Near Elevator Near Elevator Biochemistry & Immunology section Near Generator Microbiology section Near Toilet Hematology section Near RO water system Lab Office   QMS Office   DNA Extraction Room of Molecular section 1   Analysis Room of Molecular section 1   Molecular section 2   Autoclave Room   Lab Store

16. Location of Fire Hose Reels At emergency staircases of Ground floor First floor Second floor Third floor Fourth floor Fifth floor Biosafety 16

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18. General safety precautions Dos Use Laboratory coats and gloves . Wash hands Remove gloves when using the telephone Remove laboratory coat and gloves before leaving the laboratory P rotect the mouth, eyes and face where splashes may occur Secure hair and cut long fingernails C uts or broken skin are covered Keep the work area tidy, clean and free of non-essential objects G lassware must be checked before use. At the start and end of each working day, all surfaces must be swabbed down with disinfectants and record in Laboratory Working Area Cleaning L og Sheet. (SML-LAB-QAS-PR-004_Form 8) 18 Biosafety

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21. Don’ts Avoid contact of gloved hands with face. Personal clothing to protrude beyond the sleeves of the laboratory coat is not allowed. Never store food or drink, or personal items Eating, drinking, smoking, and applying cosmetics must not be performed Never put materials in mouth Don’t use portable electronic devices when not specifically required for the laboratory procedures being performed. Pipetting by mouth must be prohibited. A pipetting aid must always be used. General safety precautions 21 Biosafety

22. Precautions against Infections Treat fluids carefully to avoid spills & minimize aerosol Never re-cap, clip or remove needles from disposable syringes Dispose sharps in puncture-resistant containers, three-quarters full, never reused Decontaminate work surfaces before and after use In the event of an accident involving infected material, treatment must be obtained immediately Contaminated pipettes - completely submerged in a disinfectant (bleach) To avoid creating aerosols when drying sputum samples, prepare the sample in biosafety cabinet with proper PPE. 22 Biosafety

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24. Precautions against Chemicals Chemicals - stored in a safe, secure location, accessible only to authorized laboratory workers. Store away from direct sunlight and sources of heat. Never taste L abeled and their respective hazard information Hazardous liquids, such as acids or alkalis, must be stored below eye level. Always pour acid into water for dilution. Never the reverse. 24 Biosafety

25. Precautions against Chemicals Do not store chemicals on the floor, window ledges, or balconies. Do not use flammable substances near an open flame. Replace cover on all reagent bottles No t store in alphabetical order. Acc ording to their specific hazard(s) to prevent unintended reactions 25 Biosafety

26. Precautions against Chemicals P rocedure for the discarding and safe disposal used in the laboratory according to SDS must be maintained. Store the Material Safety Data Sheets (MSDS) in hard copy at QMS office and in soft copy in QMS team’s computer. Check regularly the MSDS Read the MSDS for information on all chemicals you plan to use. 26 Biosafety

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28. Precaution against Physical Injuries Many sharp materials are used in the medical laboratory and staffs must acquaint themselves with proper manipulation. If any accident involving even trivial personal injury, it should be reported to a person in charge so that it can be noted. All cases of burns must be referred to a doctor as frequently as possible. 28 Biosafety

29. Fire safety Training of laboratory staff in fire prevention, immediate action in case of fire and the use of fire-fighting equipment Ventilate area where flammable are used Fire-fighting equipment Fire extinguishers, smoke detectors and escape route should be regularly inspected and maintained (SML-LAB-QAS-PR-004_Form 4 Fire and electrical safety checklist) Ensure open flames or heat sources are never placed near flammable supplies and are never left unattended. Precautions against Fire and Electric Shock 29 Biosafety

30. Fire safety Flammable chemicals including benzene, xylene, acetone and alcohol must be stoppered when not in used and stored separately. Avoid strong flammable materials in direct sunlight Prohibit smoking and cooking in the laboratory Fire evacuation plan (SML-LAB-QAS-PR-004_Form 2 Fire escape plan) Clear obstructed corridors, aisles and room exits. Precautions against Fire and Electric Shock 30 Biosafety

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32. Electrical safety All electrical installations and equipment are inspected and tested regularly (SML-LAB-QAS-PR-004_Form 4 Fire and electrical safety checklist). The installation of electric equipment by the supplier of an apparatus or by the hospital electrician and a trained laboratory equipment technician. All laboratory electrical equipment should be grounded Safety awareness of the potential hazards associated with the electrical appliances and laboratory Labelling of warning signs are down when high voltage power supplies are in use 32 Biosafety

33. Electrical safety Promptly report malfunctions of electrical devices using Service Request Form (SML-LAB-QAS-PR-004_Form 10) Never operate electrical equipment with wet hand Use only approved extension cords in temporary applications and do not overload circuit To check the electrical equipment that the voltage selectors switch is in the correct position 33 Biosafety

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35. Making sure that the equipment is wired correctly. Always switch off power after using electrical equipment. Fire emergency measured should be taken in advance. It should be controlled by a dry chemical type extinguisher or dry sand. Water must never be used. Biosafety 35

36. Precautions with sample collection Gloves should be worn for all procedures. Remove gloves whenever contaminated Wash hands after removing gloves Blood should be collected by trained staff. Never re-cap, clip or remove needles Dispose of sharps in puncture-resistant containers . Containers should be correctly labelled Tightly seal the specimen containers, if outer surfaces are infected, wipe out with disinfectants. P lace in adequate containers for transport Specimen request or specification forms - placed separate, preferably waterproof bags or envelopes. 36 Biosafety

37. Use of Centrifuge Centrifuge tubes and specimen containers should be inspected for defects before use. Should be correctly balanced by using distilled water or alcohol (propanol, 70%) Should be inspected daily and decontaminated at the start and end of the working day and recorded in log sheet (SML-LAB-QAS-PR-008_Form 1 Centrifuge cleaning log sheet) 37 Biosafety

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39. Care and use of refrigerators Temperature of the refrigerators, freezers are checked daily by temperature record. All containers stored in refrigerators should be clearly labelled. Biohazard symbol must be posted No food or drink must be stored Incubator temperature are checked by temperature record. 39 Biosafety

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42. Infection tests Hepatitis A, B, C HIV Chikungunya Chlamydia trachomatis Covid-19 CMV Dengue Epstein Barr virus HSV Typhoid Influenza Leptospirosis Mycoplasma pneumoniae Malaria Microfilaria Measles Mumps Rubella VZ Syphilis Tuberculosis (TB) Toxoplasmosis Syphilis Stool infection tests 42 Biosafety

43. During sample collection During transport During analysis During housekeeping and disinfection During waste disposal During outside transport 43 Biosafety

44. Symptoms of infectious pathogens 44 Biosafety

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50. 50 Biosafety provides personnel , product , and environmental protection. It is commonly found in clinical and research laboratories There are four types (A1, A2, B1, and B2 ) The main differences between the types are the ratio of air exhausted from the BSC to the air that is recirculated within the BSC, and the type of exhaust system present. About 90% of all biosafety cabinets installed are Type A2 cabinets.  Class II BSC

51. 51 Biosafety Class I BSC the first designed and simple provides personnel and environmental protection but not product production (as unsterilized room air is drawn over the work surface). Class III BSC provides the highest level of personnel protection suitable for work in Biosafety Level 3 and 4 laboratories.

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55. Biosafety cabinet practices Before materials are introduced, decontaminate with 70% alcohol to remove any external contaminants Clean materials at one side of the work surface, dirty items on the other. Work from “clean” to dirty Cabinets should be turned on at least 5 min before beginning Avoid rapid movements. Move in a controlled and steady The chair - adjusted so that the lower portion of the sash is even with the worker’s armpits. 55 Biosafety

56. Biosafety cabinet practices Any paper or plastic materials - not interfere with air flow Decontaminate with cleaning agents after use. Germicidal UV lamps are turned on after handling of aerosol releasing samples. Disinfect items/pipettes within the cabinet while biohazard wastes must be bagged inside the cabinet Do not use the cabinet with any toxic, flammable or explosive materials. 56 Biosafety

57. Biosafety cabinet practices Do not place unnecessary items inside the cabinet. Do not operate if any of the alarms are activated. Do not use the cabinet as storage area. At the end of the work day, the final surface decontamination by 70% alcohol or bleach (second wiping with sterile water is needed). Biosafety cabinet usage is recorded in log sheet (SML-LAB-QAS-PR-004_Form 11) Regular certification 57 Biosafety

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59. Personal Protective Equipment (PPE) Any PPE used in the laboratory must be correctly fitted. When combinations of PPE are worn together, they must complement one another and continue to fit properly. PPE are kept in the biochemistry section of the laboratory. 59 Biosafety

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61. Personal Protective Equipment (PPE) 61 Biosafety

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64. Remember… Safety First! 64 Biosafety