Lab titration

Acid-Base Titration

1. STELLA MARIS POLYTECHNIC Mother Patern College Of Health Sciences Biology Department General Chemistry 101 LAB Mr. Abraham G. Dayklee Instructor BSc. Bio/Chem- MPCHS MOTHER PATERN COLLEGE OF HEALTH SCIENCES BSc. Biology/Chemistry

2. Acid & Base Titration

3. Acid-Base Titrations Acid-Base titrations are usually used to find the amount of a known acidic or basic substance through acid base reactions. The analyte (titrand) is the solution with an unknown molarity. The reagent ( titrant) is the solution with a known molarity that will react with the analyte.

4. Apparatus/Equipment

5. Determining the Concentration of a Solution of Hydrochlorolic Acid: A known volume ( perhaps 25.00ml ) of the unknown acid is measured into a flask using a Pipette.

6. Determining the Concentration of a solution of Hydrochlorolic Acid: An indicator , a substance that changes colour as the solution reaches a certain pH, is added to the unknown solution. Phenolphthalein Methyl orange

7. Determining the Concentration of a Solution of Hydrochlorolic Acid: 3. A solution of sodium hydroxide (perhaps 0.1000M) is carefully added to the unknown solution using a Burette, which is a long glass tube calibrated in millilitres. a.) A stopcock at the bottom of the buret regulates the amount of liquid dispensed b.)The standard solution is added until the indicator changes colour.

8. Determining the Concentration of a Solution of Hydrochlorolic Acid: 4. At this point, the equivalence point, the number of moles of hydroxide ion added is equal to the number of moles of hydronium ion present in the unknown acid.

9. Determining the Concentration of a Solution of Hydrochlorolic Acid: 5. The volume dispensed by the Buret (perhaps 35.00mL) is measured and used in the calculation of the unknown acid concentration. Calculation: (M acid ) (V acid ) = (M base ) (V base ) M acid = M base (V base /V acid )  M acid = (0.1000M) (35.00ML / 25.00mL) M acid = 0.1400M

10. Procedure Summary The analyte is prepared by dissolving the substance being studied into a solution. The solution is usually placed in a flask for titration. A small amount of indicator is then added into the flask along with the analyte .

11. Procedure Summary The reagent is usually placed in a burette and slowly added to the analyte and indicator mixture. The amount of reagent used is recorded when the indicator causes a change in the colour of the solution.

12. Acid-Base Neutralizing Acid-Base Neutralized

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