LABORATORY SAFETY GUIDELINES AND PPE USES.pdf

1. Personal Protective Equipment (PPE)
2. General Lab Conduct
3. Chemical Safety
4. Biological Safety
5. Equipment Safety
6. Emergency Procedures
7. Waste Disposal
8. Training and Competency
9. Documentation and Compliance
10. Communication and Reporting

1. LABORATORY
SAFETY GUIDELINES
AND PPE USES
BY: SHIVANI SHARMA
ASSISTANT PROFESSOR
GLOBAL RESEARCH INSTITUTE OF PHARMACY, RADAUR

2. Content
Personal Protective Equipment (PPE)
General Lab Conduct
Chemical Safety
Biological Safety
Equipment Safety
Emergency Procedures
Waste Disposal
Training and Competency
Documentation and Compliance
Communication and Reporting

3. Personal Protective Equipment
(PPE)
Lab Coats:Always wear a lab coat to protect your skin and clothing
from chemicals and biological materials.
Gloves:Use appropriate gloves for handling chemicals or biological
specimens. Change them frequently and avoid touching personal
items (e.g., phones) while wearing gloves.
Safety Goggles/Face Shields:Protect your eyes and face from
splashes, debris, and harmful substances.
Masks:Use masks or respirators if working with volatile chemicals or
biological agents that may be inhaled.

4. General Lab Conduct
No Eating or Drinking:Avoid consuming food or drinks in the lab to
prevent contamination.
Keep Workspace Clean:Maintain an organized and clean work
area. Clean up spills immediately and dispose of waste properly.
Follow Instructions:Adhere strictly to lab protocols and procedures
as outlined by your instructor or supervisor.
Report Hazards:Notify your supervisor immediately if you encounter
any unsafe conditions or equipment malfunctions.

5. Chemical Safety
Labeling:Ensure all chemicals are clearly labeled with their
contents, hazards, and expiration dates.
Storage:Store chemicals in designated areas according to their
compatibility and hazard classification. Keep flammable chemicals
away from heat sources.
Handling:Use appropriate techniques and equipment for handling
chemicals. Never pipette by mouth or directly inhale chemical
fumes.
Spill Response:Follow specific procedures for cleaning chemical
spills, and use spill kits if necessary. For large spills, evacuate the area
and alert emergency services.

6. Biological Safety
Containment:Use biosafety cabinets for handling potentially
infectious materials. Follow biosafety level guidelines relevant to the
agents being used.
Sterilization:Properly sterilize equipment and dispose of biological
waste in designated containers.
Personal Hygiene:Wash hands thoroughly after handling biological
materials and before leaving the lab.

7. Equipment Safety
Inspection:Check equipment for defects or malfunctions before
use. Do not use damaged equipment.
Operation:Operate equipment according to manufacturer
instructions and training. Never leave equipment running
unattended.
Maintenance:Regularly maintain and clean equipment as per lab
guidelines.

8. Emergency Procedures
Fire:Know the location of fire extinguishers and how to use them.
Familiarize yourself with the lab’s fire evacuation plan and exits.
Chemical Exposure:In case of chemical contact with skin or eyes,
immediately use the nearest eye wash station or safety shower. Seek
medical attention if needed.
First Aid:Be aware of basic first aid procedures for common lab
injuries (cuts, burns, etc.). Keep a first aid kit readily available.

9. Waste Disposal
Segregation:Separate waste into appropriate categories: chemical,
biological, and sharps. Follow lab-specific guidelines for disposal.
Containers:Use designated containers for each type of waste. Do
not overfill containers and ensure they are properly sealed before
disposal.
Disposal Procedures:Follow established procedures for disposing of
hazardous materials and report any issues with waste disposal.

10. Training and Competency
Training:Ensure all lab personnel receive proper training on safety
protocols, equipment use, and emergency procedures.
Competency:Regularly assess and refresh training to ensure
ongoing compliance with safety practices.

11. Documentation and Compliance
Safety Data Sheets (SDS):Review SDS for all chemicals and materials
used in the lab.
Record Keeping:Maintain accurate records of safety inspections,
training sessions, and incidents.
Regulations:Comply with local, state, and federal regulations
regarding laboratory safety and hazardous materials.

12. Communication and Reporting
Safety Meetings:Participate in regular safety meetings and briefings.
Incident Reporting:Report accidents, near-misses, and unsafe
conditions promptly to the appropriate authorities.

13. THANK YOU