Lc ms ms

LC MS/MS....

1. LIQUID CHROMATOGRAPHY MASS SPECTROMETER MASS SPECTROMETER LC- MS/ MS

2. INTRODUCTION liquid chromatography + mass spectrometry physical separation and mass analysis Identify variety of compounds high sensitivity and specifity

3. LIQUID CHROMATOGRAPHY HPLC Principle: Pump pass pressurized liquid solvent Sample pass through a column Each component interacts differently with adsorbent S eparation of the components

4. TANDEM MASS SPECTROMETRY MS/MS MS1: precursor ions are formed separate by m/z ratio Daughter ions are created by fragmentation MS2: Ions are separated and detected

5. TANDEM MASS SPECTROMETERY

6. COMPONENTS

7. ION SOURCE

8. ELECTRON SPRAY IONIZATION

9. ATMOSPHERIC PRESSURE PHOTO IONIZATION

10. ATMOSPHERIC PRESSURE CHEMICAL IONIZATION

11. TIME OF FLIGHT

12. QUADRUPOLE

13. ION TRAP

14. FRAGMENTATION

15. DETECTOR

16. ADVANTAGES

17. APPLICATIONS

18. WHAT IS THE DIFFERENCE BETWEEN LC-MS and LC-MS/MS ???