Leaf fingerprinting Stomata Lab presentation

Leaf fingerprinting

1. Stomata Lab
Leaf fingerprinting

2. Lab Prep
•Paint 2 cm by 2 cm square of clear nail
polish on top and bottom of leaf

3. Photosynthesis

4. Photosynthesis Equation

CO
2
+ H
2
O C
6
H
12
O
6
+ O
2
light
6 6 6

5. Light vs. Dark
LocationReactantsProducts
Light
Reaction
Thylakoid
Membrane
Sunlight
H
2O
ATP,
NADPH,
and
oxygen
Dark
Reaction
Stroma ATP,
NADPH,
CO
2
Glucose

6. Stomata
•Small pores
located on the
underside of a
leaf
•Allow
–CO
2
in
–O
2
and H
2
O
out

7. Stomata
•When are they
open?
–Day
•When are they
closed?
–Night

8. Guard Cells
•Swell: Cell’s vacuole
fills with water
–Stomata Open
–Day
•Shrink: Water leaves
cell’s vacuole
–Stomata Close
–Night

9. Where would you find
stomata on a lily pad? Why?

10. Microscope Review
•What is the correct way to hold a
microscope?
–Two hands!
–Support arm and base of microscope!
•What objective lens do I use to first
focus an image?
–low

11. Microscope Review
•When do you use the coarse adjustment
knob?
–ONLY ON LOW
–NEVER TOUCH COARSE ADJUSTMENT ON
ANY OTHER OBJECTIVES!
•If I am looking at an image on the
40x objective lens, what is my TOTAL
magnification?
–40x objective x 10x ocular = 400x total
magnification

12. Stomata Lab
•Label: stomata, guard cells, total magnification
HIGH Power LOW Power

13. Stomata Lab
•FOV (Field of View): # of stomata you
can see
LOW Power6