Magnetic Bead-Based Nucleic Acid Isolation.pdf

Hello everyone in our course.
It is Level One Episode 6 in the practical molecular biology course from A to Z.
In this pdf, we will discover:
Introduction to the Magnetic Bead-based Nucleic Acid Isolation method
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Four-stage process:
1) Binding target biomolecule (DNA or RNA) with beads
2) immobilization through applying an external magnetic field
3)Washing
4)Elution

1. Magnetic Bead-Based
Nucleic Acid Isolation

2. Paperclip
Magnetic Bead-Based
Nucleic Acid Isolation

3. Advantages
high yield economical user-friendly nature
the possibility of
automated process
lesser chance of
cross-contamination
due to the exclusion
of harmful chemicals
significant scalability

4. Magnetic Beads
•Magnetic beads are small nano- or micro-sized particles studied as
potential ways to solve scalability issues regarding nucleic acid
isolation, extraction, and manipulation
•These beads can be quickly immobilized and resuspended by applying
alternate magnetic fields, making them a promising candidate for
nucleic acid extraction.
•Depends on paramagnetism and superparamagnetism
•Prepared from particles such as FeO4

5. Stages of extraction process
binding target
biomolecule with
beads
immobilization
through applying
external
magnetic field
washing Elution

6. binding target
biomolecule with beads

7. Stages of extraction process
binding target
biomolecule with
beads
immobilization
through applying
external
magnetic field
washing Elution

8. immobilization through
applying external magnetic
field

9. immobilization through
applying external magnetic
field
washing

10. Stages of extraction process
binding target
biomolecule with
beads
immobilization
through applying
external
magnetic field
washing Elution

11. Elution

13. Applications
genetic
engineering
hereditary and
genomic
identification
diagnosis
pharmaceuticals
vaccine
development

15. References
[1] S. Berensmeier, “Magnetic particles for the separation and purification of nucleic acids,” Appl. Microbiol. Biotechnol., vol.
73, no. 3, pp. 495–504, 2006, doi: 10.1007/s00253-006-0675-0.
[2] D. Horák, M. Babič, H. Macková, and M. J. Beneš, “Preparation and properties of magnetic nano- and microsized particles
for biological and environmental separations,” J. Sep. Sci., vol. 30, no. 11, pp. 1751–1772, 2007, doi: 10.1002/jssc.200700088.
[3] K. R. Paithankar et al., “Us5898071,” vol. 9, no. 19, 1999.
[4] N. Kovačević, “Magnetic Beads Based Nucleic Acid Purification for Molecular Biology Applications,” pp. 53–67, 2016, doi:
10.1007/978-1-4939-3185-9_5.
[5] A. Sebastianelli, T. Sen, and I. J. Bruce, “Extraction of DNA from soil using nanoparticles by magnetic bioseparation,” Lett.
Appl. Microbiol., vol. 46, no. 4, pp. 488–491, 2008, doi: 10.1111/j.1472-765X.2008.02343.x.
[6] A. Gautam, “Magnetic Bead-Based Nucleic Acid Isolation.” pp. 111–117, 2022. doi: 10.1007/978-3-030-94230-4_15.
[7] Y. Haddad, K. Xhaxhiu, P. Kopel, D. Hynek, O. Zitka, and V. Adam, “The isolation of DNA by polycharged magnetic
particles: An analysis of the interaction by zeta potential and particle size,” Int. J. Mol. Sci., vol. 17, no. 4, pp. 1–11, 2016, doi:
10.3390/ijms17040550.
[8] K. Rudi, M. Kroken, O. J. Dahlberg, A. Deggerdal, K. S. Jakobsen, and F. Larsen, “Rapid, universal method to isolate PCR-
ready DNA using magnetic beads,” Biotechniques, vol. 22, no. 3, pp. 506–511, 1997, doi: 10.2144/97223rr01.
•Recommended videos:
•https://youtu.be/tKx_L6G81NU?si=9q8G2mQsD9XPTD7k
•https://youtu.be/YI_csEPU154?si=qcatVoQXqGFpm5nS
•Link for PCR purification:
https://www.integra-biosciences.com/global/en/applications/beckman-coulter-ampure-xp-beads-protocol-pcr-purification