Materials_and_Methods_Polyalthia_longifolia.pptx

Abdominal fat, Broiler chickens, Carcass traits, Leaf meal supplementation and Polyalthia longifolia.

1. MATERIALS AND METHODS Research on the Effect of Polyalthia longifolia Leaf Meal on Broiler Performance

2. 3.1 Place and Time The research was conducted for 5 weeks at the Fapet Farm, Faculty of Animal Husbandry, Jambi University.

3. 3.2 Materials and Equipment • Materials: 200 one-day-old broiler chicks (DOCs). • Feed ingredients: Corn, bran, soybean meal, fish meal, oil, lysine, methionine, minerals, premix, and Polyalthia longifolia. • Equipment: 20 cages, lamps, feed and water containers, digital scales, knives, and other supporting tools.

4. 3.3 Methods Overview • Sanitation was performed using disinfectants before chick placement. • A total of 200 DOCs were allocated into cages (10 birds each). • The experiment lasted for 5 weeks with diets containing 0%, 2%, 4%, and 6% Polyalthia longifolia leaf meal.

5. 3.3.1 Cage Preparation • Cages were disinfected and dried before chick placement. • Lime was applied to the cage floor for one week to break disease cycles. • Feeders, drinkers, and lamps were installed and sanitized. • Each cage housed 10 chicks randomly assigned to treatments.

6. 3.3.2 Ration Preparation • Diets included corn, fish meal, rice bran, soybean meal, premix, oil, lysine, methionine, and Polyalthia longifolia. • Polyalthia longifolia leaves were air-dried and ground into fine powder. • Feed was mixed thoroughly to ensure homogeneity. • Nutrient formulation followed NRC (1994) standards.

7. Treatment Groups P0 = 100% basal diet + 0% Polyalthia longifolia P1 = 100% basal diet + 2% Polyalthia longifolia P2 = 100% basal diet + 4% Polyalthia longifolia P3 = 100% basal diet + 6% Polyalthia longifolia

8. 3.3.3 Research Implementation • Chickens and feed treatments were randomized across 20 cage units. • Body weights were recorded weekly after 8 hours of fasting. • Feed consumption was measured and averaged weekly.

9. 3.4 Research Design A Completely Randomized Design (CRD) was used with 4 treatments and 5 replications. Each replication contained 10 birds. Model: Yij = μ + πi + εij Where: Yij = Observation value μ = General mean πi = Treatment effect εij = Experimental error

10. 3.5 Observed Variables Observed parameters: • Feed intake • Body weight gain • Feed conversion ratio (FCR) • Slaughter weight • Carcass weight • Relative carcass weight

11. 3.6 Data Analysis Data were analyzed using Analysis of Variance (ANOVA) according to the Completely Randomized Design (CRD). Significant differences among treatments were determined at P<0.05 using Duncan’s Multiple Range Test.