Mc fadyean reaction

anthrax staining diagnosis

1. Mc’Fadyean Reaction ABHIJITH SP CVAS POOKODE

2. INTRODUCTION Used to identify Anthrax bacillus in microscope. Actually a Staining technique. Employed for the presumptive diagnosis of anthrax in animals. Stains the polypeptide capsular material of anthrax bacilli that is composed of poly-d–Glutamic Acid.

3. PROCEDURE Take blood from tip of ear and make a impression slide. This is stained with Poly Chrome Methylene Blue (PCMB ) for 30 seconds. Examine under microscope. If the sample is positive, an amorphous purplish material is noticed around the blue bacilli. This purplish material is capsule around blue bacilli body.

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