Microbial enzymes

Presentation about enzymes especially enzymes produced from microorganisms, their production process, and most important enzymes.
* Made by prezi

1. Microbial Enzymes

By/ Mai M.ElFouly

2. Microbial Enzymes

By/ Mai M.ElFouly

3. Enzymes

Immobilization o
Enzymes

Hypothesis of
enzymes

Inhibition of
enzyme activity

+ competitive inhibition
+ Noncompetitive inhibition
+ Uncompetitive inhibition:
+ Feedback inhibition
+ Allosteric regulation

4. Hypothesis of
enzymes

- lock and Ki

Prezi

6. Immobilization o
Enzymes

7. Enzyme specificity

1-Relative, low or bond specificity
2-Moderate, structural or group specificity
3-Absolute, high or substrate specificity
4-Optical or stereo - specificity

5-Dual specificity

8. actors affecting enzyme actio

- Enzyme concentration
- Substrate concentration

+ Temperature

+ PH

- Time

« Concentration of coenzymes
« Concentration of metal ions
- Presence of inhibitors

9. Advantages of microbial
enzymes

- broad range of PH,
temp and substrate

+ specificity

- Variety of enzymes

- time and quantity

- production cost

- biodegradable

10. Production Of

Enzymes ue

Enzymes: Production of
microbial enzymes

How bacteria can adapt to
environmental changes?

‘Mutation

Toxins
Antibiotics:

+ tone bac om eae ce.
“esting gr parameters anc orden

- Selecting the righ enzyme.
‘Fang gene

| Transtering genet produce orgasism
Design he rosin eto

Fermentation

11. How bacteria can adapt to
environmental changes?

Mutation

Toxins

Antibiotics

Enzymes

12. Production of
microbial enzymes

- Isolation of bacteria from exotic places.
- Testing growth parameters and conditions
+ Selecting the right enzyme.

- Finding gene

- Transferring gene to productive organism
- Developing the production method

+ Fermentation

13. Fermentation

AR
El. ¿Es E. (EEE)

Preserved Inoculum Inoculation Fermenter
inoculum development tank

| al

(Enzyme k—{ Freeze Final purification Filtration Cool Salt treatment )
(powder drying (chromatography etc.) storage ah

Fig. 21.1 : An outline of the flow chart for the production of enzymes by microorganisms.

15. Endoenzymes

- Taq Polymerase
- Luciferase

16. Glowing Plant
Project

17. Exoenzymes

+ Protease — Lipase ... Detergents
and leathers industry

- Cellulase — Amylase ... paper
industry

18. THANK YOU

19. Microbial Enzymes

By/ Mai M.ElFouly