MICROBIOLOGY-PARASITOLOGY-LABORATORY-SAFETY-AND-RULES.pptx

Laboratory Safety and Rules

1. MICROBIOLOGY -PARASITOLOGY LABORATORY SAFETY PROTOCOLS GENERAL SCIENCE LABORATORY

2. Why do we need to conduct this orientation? Potential exposure to infectious agents: Working in microbiology involves handling various microorganisms, some of which can be highly pathogenic. Contamination risks: Microbiology experiments require aseptic techniques to prevent contamination of samples. Be meticulous in maintaining cleanliness and following sterile procedures to ensure accurate results and prevent cross-contamination. Biohazardous waste management: Proper disposal of biohazardous waste is crucial in microbiology. Follow the guidelines provided by your institution or laboratory to ensure safe handling and disposal of infectious materials. Ethical considerations: Ethical considerations may arise when conducting research or experiments. Stay informed about ethical guidelines and regulations relevant to your work and ensure that your practices align with responsible scientific conduct.

3. RULES BEFORE CONDUCTING THE EXPERIMENT: Place all bags in the bag stations, never bring inside your gadgets, foods, and drinks. Sanitize your hands before entering the laboratory. Wear your laboratory gowns, gloves, hair net, and face mask. (NO PPE’s, NO LABORATORY EXPERIMENT) Proper segregation and disposal of BIOWASTE must be observed all the time. Sanitize your workplace before performing the activity. Must have garbage bag inside before the activity.

4. RULES DURING THE CONDUCT OF THE EXPERIMENT: It is imperative to listen attentively to your instructors. Your safety and the safety of everyone is your RESPONSIBILITY. Avoid unnecessary chatting. Never touch your face, doorknobs, TV, windows with your gloves on. If you need to leave the room, remove your PPE, disinfect your hands and use another PPE when your resume. Never place broken glassware inside the incubator and autoclave. Put label to your outputs if it requires, overnight observation. Place it in the holding and observation room.

5. 7. Have a ready disposal bags/bins in the laboratory. Segregate properly: (1) for the disposal of PPE’s, (2) for BIOWASTE 8. EVERY CLASS MUST HAVE DISINFECTANTS (Before the start of class, per sections should provide alcohol. Label your materials and give to your instructors, this will be use only by your sections everytime you will conduct your activity> 70% isopropyl alcohol is preferred ) 9. Tissues/wipes used in disinfecting the tables and chairs must be DISPOSED. 10. Give a least 20 minutes time to clean and disinfect your areas. You can use a mixture of 240 mL of bleach mixed with 5 gallons of water as disinfectant and allow air dry.

6. RULES AFTER THE CONDUCT OF ACTIVITY: Never leave the room with your PPE on. Removal of PPEs should be done inside the lab. Disinfect your hands using an alcohol. ENSURE TO CLEAN THE LABORATORY BEFORE LEAVING. FAILURE TO DO WILL RENDER 1 WEEK COMMUNITY SERVICE IN THE LABORATORY AND WILL BE REPORTED TO THE OSS. Turn off the lights, TV, and exhaust fan. Leave the windows open for ventilation.

7. The proper disposal of laboratory waste in the Philippines is governed by various laws and regulation. Here are the key regulations related to laboratory waste disposal in the Philippines: 1. Ecological Solid Waste Management Act (Republic Act No. 9003): This law provides the framework for the proper management of solid waste, including laboratory waste. It promotes waste segregation, recycling, composting, and the establishment of appropriate disposal facilities. It also encourages the adoption of environmentally sound technologies and practices for waste management

8. 2 . Hazardous Waste Management Act (Republic Act No. 6969): This act focuses on the proper handling, transport, treatment, storage, and disposal of hazardous waste, which includes certain types of laboratory waste. It aims to prevent pollution and protect human health and the environment from the risks associated with hazardous substances.

9. LABORATORY SAFETY IS THE RESPONSIBILITY OF EVERYONE

10. Your cooperation will be highly appreciated. This orientation may serve as an agreement between you and the teachers to follow and abide by the protocols of the laboratory for everyone’s safety.