Microscope.pptx

microscope its parts variables history and discovery microscopy types of a microscope and its care

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2. microscope Contents History and discovery Microscopy Variables used in microscopy What is a microscope? Types of microscope Parts of a microscope

3. H istory of the microscope Roman philosophers mentioned “burning glasses” in their writings but the first primitive microscope was not made until the late 1300’s . in 1609 Galileo Galilei perfected the first device known as a microscope Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.

4. Leeuwenhoek is considered the founder of the study of microscopy and an played a vital role in the development of  cell theory .

5. Microscopy Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye)

6. Variables in Microscopy Magnification Degree of enlargement No of times the length , breath or diameter of an object is multiplied

7. Resolution Ability to reveal closely adjacent structural details as separate and distinct Limit of resolution (LR)

8. Contrast Difference in intensity between two objects, or between an object and background

9. Working distance Distance between the front surface of lens and surface of cover glass or specimen

10. What is a microscope? microscope, instrument that produces enlarged images of small objects, allowing the observer an exceedingly close view of minute structures at a scale convenient for examination and analysis

11. Types of Microscope Light microscope A light microscope is a biology laboratory instrument or tool, that uses visible light to detect and magnify very small objects and enlarge them. They use lenses to focus light on the specimen, magnifying it thus producing an image. The specimen is normally placed close to the microscopic lens.

12. Compound microscope A compound microscope is a high power magnification ) that uses a compound lens system . T he   objective lens   and   eyepiece lens Higher magnification is achieved by using two lenses rather than just a single magnifying lens.

13. Electron microscope An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination . There are two types of electron microscope: Scanning electron microscope Transmission electron microscope

14. Scanning electron microscope scanning electron microscope (SEM), type of  electron microscope , designed for directly studying the surfaces of solid objects, that utilizes a beam of focused  electrons  of relatively low  energy  as an electron probe that is scanned in a regular manner over the specimen.

15. Transmission electron microscope Transmission electron microscopes (TEM) are microscopes that use a particle beam of electrons to visualize specimens and generate a highly-magnified image. TEMs can magnify objects up to 2 million times

16. Parts of a microscope Ocular lens Arm Stage Corse adjustment knob Fine adjustment knob Base Body tube Revolving nosepiece Objective lens Stage clips Diaphragm light

17. Microscope care Always use two hands when moving the microscope. Make sure the microscope is not plugged in before moving it. If using  immersion oil , be sure to  clean the immersion oil lens  thoroughly after use. Keeping your microscope clean  (especially the optics) will also ensure that it lasts longer. When the microscope is not in use cover it with a  dust cover or store it in a microscope case .

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