modern biology presentation used for research (1).pptx

talks about new methodologies in the biological field used for research

1. New methodologies in biological field

2. Introduction The balance between melanin synthesis and melanin degradation is important in the homeostasis of skin colour

3. Cell Viability: MTT Assay The principle: only viable cells have active metabolism and can convert yellow MTT to purple formazan , which has an absorbance maximum at 570nm. A mount of formazan proportional to the number of viable cells Results 1.A. fruticosa RE Induces Depigmentation in α-MSH-Stimulated B16F10 Cells

4. B.)RNA extraction

5. A) .Western Blotting Separation of Proteins based on their based on molecular weight, 2.A. fruticosa RE-Mediated Depigmentation Depends on Autopagy

6. c.)Confocal Microscopy  a specialized form of standard fluorescence microscopy that uses optical components to generate high-resolution images of material stained with fluorescent probes. Pinhole- only lets a very small part of image through at a time thus allowing for better filtration of photon emitted by labeled structure from any background fluorescence.

7. 3Identification of Amorphigenin as a Functional Metabolite in A. fruticosa RE Method

8. Amorphigenin -Mediated Depigmentation Depends on Autophagy

9. Amorphigenin Induces Degradation of Melanosomes in an mTOR-Independent and AMPK Dependent manner

10. In summary ; In the realm of cellular immunology, a range of advanced techniques is used . Flow cytometry and cell cultures identify and study immune cell behaviours. Advanced imaging and high-throughput screening capture cell interactions and aid drug discovery. Omics technologies offer comprehensive insights, while 3D models simulate real conditions. These methods collectively deepen our understanding of immune cells, fostering progress in therapies and treatments.