Monoclonal antibodies production slideshare

Definition
Methods
Monoclonal Abs Production
Application

1. MONOCLONAL ANTIBODIES PRODUCTION

2. Monoclonal Antibodies are the antibodies that are made by identical immune cells that are all clones of a unique parent cell. Monoclonal antibodies can have monovalent affinity, in that they bind to the same epitope. A technique to produce monoclonal antibodies was devised by Georges Kohler and Cesar Milstein in 1975 . DEFINITION

3. METHOD Immunization of a mouse Isolation of B-cell from the spleen Cultivation of myeloma cells Fusion of myeloma and B cells Separation of cell lines Screening of suitable cell linese In vitro or in vivo multiplication harvesting

4. MONOCLONAL ANTIBODY PRODUCTION Monoclonal antibodies (mAbs) are produced in vitro using Tissue - culture techniques. A mouse is injected with a specific antigen that will induce antibodies against that antigen. The spleen of the mouse is removed and homogenized into a cell suspension. The suspension includes B - cells that produce antibodies against the injected antigen.

5. The spleen cells are then mixed with Myeloma cells (Cancerous B - cells) , That are capable of continuous growth in culture but have lost the ability to produce antibodies. Some of the antibody-producing spleen cells and myeloma cells fuse to form hybrid cells. These hybrid cells are now capable of growing continuously in culture while producing antibodies.

6. The mixture of cells is placed in a Selective medium that allows only hybrid cells to grow. Hybrid cells proliferate into clones called Hybridomas . The Hybridomas are screened for production of the desired Antibody. The selected Hybridomas are then cultured to produce large quantities of Monoclonal Antibodies.

7. This is a very expensive and time-consuming process. It may take weeks of culturing, And many liters of media to provide enough mAbs for an experiment or to treat a single patient. mAbs are expensive

8. Applications of Monoclonal Antibodies 1) Diagnostic tests – Western blot, Immuno dot blot and Immunofluorescence test. 2) Analytic and chemical use – Immunoprecipitation . 3) Therapeutic treatment. 4) Cancer Therapy. 5) Autoimmune disease Therapy.

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