Multiplex pcr

Multiplex PCR is a technique whereby PCR is used to amplify several different DNA sequences simultaneously. It is a type of target enrichment approach. It was first described in 1988 as a method to detect deletion mutations in the dystrophin gene – the largest known human gene

1. Prepared by: Anfal Ibrahim Baher Eldeen Multiplex PCR

2. Contents Introduction Types Multiplex PCR primer design Advantages Applications

3. I ntroduction

4. Types of Multiplex PCR 1 . Single Template PCR Reaction This technique uses a single template which can be a genomic DNA along with several pairs of forward and reverse primers to amplify specific regions within a template . 2. Multiple Template PCR Reaction It uses multiple templates and several primer sets in the same reaction tube.  

5. Types of Multiplex PCR Qualitative Quantities

6. Primer design – work plan

7. Examples of multiplex PCR primer design software PrimerPlex is an efficient tool to design specific oligos for multiplex PCR assays. Design Real time Multiplex PCR Primers using Beacon Designer and AlleleID ®

8. Good multiplex PCR primers properties

9. 0ptmising multiplex PCR condition

10. Multiplex Real time PCR

11. Example of stander multiplex PCR

12.   Advantages of Multiplex PCR

13. Applications of Multiplex PCR Pathogen Identification   High Throughput SNP Genotyping   Mutation Analysis   Gene Deletion Analysis   Template Quantitation   Linkage Analysis   RNA Detection Forensic Studies

14. Welcome for any quations or additions.