NANOPARTICLE

This Slide is prepared by MD SADDAM HUSSAIN

1. Presented by
MD SADDAM HUSSAIN
M PHARM (2
nd
sem)
Roll No. 05

2. CONTENTS:
INTRODUCTION
CONCEPT
CLASSIFICATION
POLYMERS FOR NANOPARTICLE
ADVANTAGES AND DISADVANTAGES
IDEAL CHARACTERICTICS
METHOD OF PREPARATION
CHARACTERIZATION AND EVALUATION
APPLICATIONS
CONCLUSION
REFERENCE

3. INTRODUCTION:
DEFINATION:
Nanoparticlesare sub-nanosized colloidal
structures composed of synthetic or semi-synthetic
polymers.”
Size range : 10–1000 nm .
The drug is dissolved, entrapped, encapsulated or
attached to a nanoparticlematrix.

4. CONCEPT:
The basic Concept involved is :
Nanoparticlesare mainly taken by :
» ReticuloEndothelial System (RES), After the administration;
» Hence are useful to carry drugs to the liver and to cells that are
phagocyticallyactive.
Control the particle size, surface properties and release of
pharmacologically active agents in order to achieve the site-
specific action of the drug at the therapeutically optimal rate and
dose regimen.

5. Classification Of Nanoparticles:
Solid Lipid Nanoparticles
Polymeric Nanoparticles
Ceramic Nanoparticles
HydrogelNanoparticles
Copolymerized Peptide Nanoparticles
Nanocrystalsand Nanosuspensions
Functionalized Nanocarriers
Nanospheres
Nanocapsules

6. Polymers For Nanoparticles:
Natural hydrophilic polymers:
•Proteins: -Gelatin, albumin, lectins, legume.
•Polysaccharides: -alginate, dextran, chitosan, agarose.
Synthetic hydrophobic polymers
•Pre-polymerized polymers: Poly (Lactic acid), Polystyrene.
•Polymerized in process polymers: Poly (isobutyl cyanoacrylates)

7. Advantages Of Nanoparticle:
Nanoparticlecan be administered by
parenteral,
oral,
nasal,
, occularroutes.
Improves stability and therapeutics index and reduce toxic affects.
Both active & passive drug targettingcan be achieved.
Reduction in the frequency of the dosages taken by the patient.
Avoids hepatic first pass metabolism.

8. Disadvantage Of Nanoparticle:
Toxic metabolites may form.
Limited drug loading.
Small size & large surface area can lead to particle
aggregation .
Physical handling of nanoparticlesis difficult in liquid and
dry forms.
High cost.

9. IDEAL CHARACTERISTICS:
It should be biochemical inert , non toxic and non-
immunogenic.
It should be stable both physically and chemically in Invivo
& invitroconditions
Restrict drug distribution to non-target cells or tissues or
organs & should have uniform distribution.
Controllable & Predicate rate of drug release.
simple, reproducible & cost effective.

10. Method Of Preparation:
A) Amphiphilicmacromolecule cross linking
1)Heat cross linking/Chemical crosslinking
2)Emulsion chemical dehydration
B) : Polymerization based
1)Emulsion (micellar) polymerization
2) Dispersion polymerization
3) Interfacial condensation polymerization

11. C) Polymerization precipitation method
1) Solvent extraction/evaporation
2) Solvent displacement(Nanoprecipitation)
3) Salting out

12. A) Nanoparticlepreparation by cross-linking of
Amphiphilicmacromolecule :
Nanoparticlescan be prepared from Amphiphilic
macromolecules, proteins and polysaccharides (which have
affinity for aqueous and lipid solvents).
The method involves Aggregation of Amphiphilesfollowed
by stabilization either by heat denaturationor chemical
cross-linkining

13. Heat cross linking / Chemical cross linking
The cross linking method is exhaustively used for nano–
encapsulation of drug.
»This method involve emulsification of bovine serum
albumin (BSA) OR human serum albumin (HSA) or
protein aqueous solution in oil using high-pressure
homogenization or high frequency sonication.

15. Emulsion chemical dehydration:
Stabilization can also be achieved by emulsion chemical
dehydration.
» Hydroxypropylcellulose solution in chloroform is used
as a continuous phase
» 2,2, di-methyl propane (Dehydrating agent) is used to
disperse into the internal aqueous phase to form an
Emulsion.
Produce nanoparticleof size ( 300 nm ).

16. B) : Polymerization based method:
1) Emulsion polymerization :
» IT CONSISTS OF :
A] Micellarnucleation and polymerization : Monomer is
insoluble in continuous phase.(O/W phase) Aqueous
phase
B] Homogenous nucleation and polymerization : Monomer
is soluble in continuous phase.(W/O phase) Organic
phase.

17. A] Micellarnucleation and polymerization
In this the monomer is emulsified in non-solvent phase
using surfactant molecule.
This lead to the formation of :
•Monomer –swollen micelle
•Stabilised monomer droplet
•The monomer molecule reaches the micelle by diffusion
from the monomer droplets through continuous phase,
thus allowing polymerization to progress within micelle.
Here monomer droplet act as a reservoirs of monomer.

19. B] Homogenous nucleation and polymerization
In this both micelle and droplet act as monomer reservoir
throughtpolymer chain length . When oligomersreach
certain length, they precipitate and form primary particle
and stabilized by surfactant molecules provided by miceiie
and droplets in which the drug will entrapped to form
nanoparticles.

21. Dispersion polymerization
In emulsion polymerization , monomer is emulsified in
non –sollventphase by means of surfactants .In case of
dispersion polymerization ,monomer is dissolved on
aqueous medium.
The nucleation is directly induced in aqueous monomer
solution and presence of stabilizer or surfactant is not
necessary for formulation of stable nanospheres.

23. Interfacial polymerization:
In this method ,a polymer that becomes core of
nanoparticleand molecule to be loaded is dissolved in
volatile solvent.
Solution is then placed in to a non-solvent for both
polymer and core phase.
polymer phase is separated at o/w interface. Resultant
mixture instantly turns to milky owing to formulation of
nanocapsules.

25. C) Polymerization precipitation method:
1) Solvent extraction/evaporation:
This method involves the formation of o/w emulsion
between partially water miscible solvent containing
the polymer and the drug ,and aqueous phase
containing the stabilizer.
The first step requires emulsification of the
polymer solution into an aqueous phase. During the
second step polymer solvent is evaporated, inducing
polymer precipitation as nanospheres

27. Salting Out Method
Salting-out is based on the separation of a water
miscible solvent from aqueous solution via a
salting-out effect.
Polymer and drug are initially dissolved in a
solvent which is subsequently emulsified into an
aqueous gel containing the saltingoutagent
(electrolytes,)

29. Solvent Displacement / Precipitation
method:
Solvent displacement involves the precipitation of
a preformed polymer from an organic solution and
the diffusion of the organic solvent in the aqueous
medium in the presence or absence of surfactant.
The solution is then poured or injected into an
aqueous solution containing stabilizer under
magnetic stirring. Nanoparticlesare formed
instantaneously by the rapid solvent diffusion.

31. CHARACTERIZATION AND EVALUATION
PARAMETER
1) Particle size spectroscopy
2) Molecular weight
3) Charge determination
METHOD
Photon correlation spectroscopy
-Laser defractometry
-Scanning electron microscopy
2) -Gel Chromatography
3)-Laser Doppler Anemometry
-Zeta potentiometer

32. 4) Density
5) Crystallinity
6) Hydrophobicity
7) Surface element analysis
-Helium compression
pynometry
-X-ray diffraction &
Differential scanning
Calorimetry,
Thermogravimetry
-Hydrophobic
interaction,
Chromatography
-X-ray, photon electron

34. APPLICATIONS:
1) Widely used in case of Cancer Therapy.
2) In lntracellularTargeting
3) Used for Prolonged Systemic Circulation.
4) As a Vaccine Adjuvant
.
5) In Case of Ocular delivery
.
6) Used in DNA Delivery.
7) Enzyme immunoassays
8) To cross BBB.

35. CONCLUSION
The sustained drug release allows pharmacological
effects to be extended due to the prolonged presence
tine of the carrier system at the targeted inflammed
area.
This new delivery system allows the desired drug to
accumulate in the inflammedtissue with high
efficiency.

36. REFERENCE:
» Targettedand controlled drug delivery by S.P . VYAS and
R.K. KHAR
» Jain N.K. “Advances in controlled and novel Drug
Delivery”, CBS publisher & Distributers, Edition 1st 2001,.
» Nanotechnology in drug delivery -A Review, Indian
Drugs, Issue 11,november 2011.
» http://en.wikipedia.org/wiki/Nanoparticle
http://www.pharmainfo.net/reviews/nanoparticles-review

37. THANK YOU