Parasitology Lab 7777777777777777777777.ppt

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1. Parasitology Lab. 7
Protozoology:
Amoebae:
Entamoeba histolytica
Entamoeba coli

2. PROTOZOA
SPOROZOA
SARCODINA
MASTIGOPHORA
CILIOPHORA
UNCERTAIN
STATUS
Phylum
Subphylum
RhizopodaClass
Entamoeba histolytica
Entamoeba coli
Endolimax nana
Iodamoeba butschlii

5. Trophozoite
Trophozoite of Entamoeba histolytica shows finger like psuedopodia and
visible fine chromatin granule lining the nuclear membrane

6. The presence of RBC's in an amoebic
field is frequently considered
presumptive evidence that the amoeba
is pathogenic and invasive (amoebic
dysentery).

8. The number of the nuclei in cyst stage is four.
The karyosome is relatively small and centrally
located,
The peripheral nuclear chromatin is relatively
fine and evenly distributed

11. 
The trophozoites may penetrate through
the muscularis into the submucosa
causing a "flask-shaped" ulcer. The
bleeding that is characteristic of the acute
infection is due to the rupture of small
blood vessels

12. Diagnosis

13. Entamoeba coli

15. Entamoeba coli
The location of the karyosome is
eccentric.
 Nucleus (single) rounded and
the nuclear membrane is lined
with coarse, irregularly
distributed chromatin granules.
 Ectoplasm is poorly
differentiated from endoplasm.
 Numerous food vacuoles
containing bacteria and fecal
particles but not RBC's.

21. Diagnosis

22. GIARDIA LAMBLIA

24. •Trophozoite
•The trophozoite has a falling leaf-like motility,
•Shape:
•In front view, it is pear shaped (or tear drop or tennis
racket shaped) with rounded anterior end
and pointed posterior end
•It is convex dorsally while the ventral surface
has a concavity bearing a bilobed adhesive disc.

25. •Trophozoite is bilaterally symmetrical; on each
side from the midline it bears
 One pair of nuclei
Pair of median bodies
Four pairs of basal bodies or blepharoplasty
(from which the axoneme arises)
Four pairs of flagella—two lateral, one ventral
and one caudal pair of flagella
Pair of parabasal bodies (connected to basal
bodies through which the axoneme passes)
Pair of axoneme or axostyle (the intracellular
portion of the flagella).

30. Diagnosis

31. Entero-test equipment showing duodenal
capsule attached with thread at other end
Cytosponge

32. TRICHOMONAS
VAGINALIS

38. Naegleria fowleri

41. Naegleria fowleri

42. Naegleria fowleri has 3 stages in its life cycle: cyst (1),
trophozoite (2), and flagellate (3) .
The only infective stage of the ameba is the trophozoite.
Trophozoites are long with a granular appearance and a single
nucleus. The trophozoites replicate by binary division during
which the nuclear membrane remains intact (a process called
promitosis) (4). Trophozoites infect humans or animals by
penetrating the nasal tissue (5) and migrating to the brain (6) via
the olfactory nerves causing primary amebic
meningoencephalitis (PAM).

43. Primary Amebic Meningoence
phalitis (PAM)