Penicillin fermentation- Pharmaceutical Microbiology

Penicillin is a antibiotic having β-lactum thiazolidine ring.

Penicillin G is the starting material for 6-amino penicillanic acid which is the key intermediate in the production of wide range of semi synthetic penicillins.

Mold used for the production of penicillin is Penicillium chrysogenum
Penicillin is a antibiotic having β-lactum thiazolidine ring.

Penicillin G is the starting material for 6-amino penicillanic acid which is the key intermediate in the production of wide range of semi synthetic penicillins.

Mold used for the production of penicillin is Penicillium chrysogenum
types of fermentation
Basically ,there are two methods of preparation of penicillin by fermentation:
Surface Culture Method:
In this method mold is grown on the surface of shallow layers of the fermentation medium.
Submerged Culture Method:
In this method mold is grown submerged in the fermentation medium.

master stock culture
It can be preserved by
Freeze drying
Fixing on soil sand mixtures
Storage under liquid nitrogen
Second method is the best method

1. Production Of Penicillin by Fermentation By- SANCHIT DHANKHAR

2. penicillin Penicillin is a antibiotic having β - lactum thiazolidine ring. Penicillin G is the starting material for 6-amino penicillanic acid which is the key intermediate in the production of wide range of semi synthetic penicillins . Mold used for the production of penicillin is Penicillium chrysogenum

3. Semi-Synthetic Prepration Of Penicillin : Pencillin is prepared semi-synthetically with the help of 6-aminopenicillanic acid(6-APA). Penicillium Chrysogenum Penicillin G Penicillin V 6-APA Apalcillin Azidocillin Ampicillin

4. Types Of Fermentation: Basically ,there are two methods of preparation of penicillin by fermentation: Surface Culture Method: In this method mold is grown on the surface of shallow layers of the fermentation medium. Submerged Culture Method: In this method mold is grown submerged in the fermentation medium.

5. Master stock culture It can be preserved by Freeze drying Fixing on soil sand mixtures Storage under liquid nitrogen Second method is the best method

6. Media: It involves two media: 1)Seed Stage Media 2)Production Stage Media Composition Of Media : Ingredients Concentration(g/l) Cornsteep Liquor 22.88 Ammonium Sulphate 10.70 Potassium dihydrozen phosphate 2.74 Sodium phenyl acetate 15.90 Glucose 81.90 Magnesium Sulphate q.s

7. Raw materials Cornsteep liquor- source of protein obtained from groundnut and cotton seed Ammonium sulphate as a source of nitrogen Potassium dihydrogen phosphate- as a source of potassium and phosphate Sodium phenyl acetate- precursor Glucose-source of carbon/sugar Magnesium sulphate /calcium carbonate as buffering agents

8. Condition Of Fermentation: Temperature: 25°C pH:5-7.5 Buffering Agent: Calcium Carbonate Antifoam Agent: Tributyl Citrate Aeration : by the help of a pipe/multiple or flat bladed disc turbines, aeration is important for the growth of mold.

9. P.chryasogenum Flask Culture Filtrate Spore Suspension Fermenter Seed Tank Washing/Filter. Distillation Rich Aqueous sol . Rich Solvent Crystalline mass Drying Penicillin G

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