Pentose Phosphate Pathway and its application

Pentose Phosphate Pathway and its significance

1. Pentose Phosphate Pathway and its application Supervised by- Mr. Tridip Boruah Assistant Professor M.C. College , Barpeta Presented by- Rakhi Sutradhar M.Sc . 2 nd Semester Roll no. : PS-231-130-0004

2. INTRODUCTION The Pentose Phosphate Pathway is a metabolic pathway parallel to glycolysis which generates NADPH and pentoses (5-carbon sugars) as well as Ribose 5-phosphate Pentose phosphate Pathway is also called Hexose monophosphate Shunt pathway. It is an alternative route for the metabolism of glucose. It is more complex pathway than glycolysis . It takes place in cytosol .

3. Phases in PPP pathway Pentose Phosphate pathway starts with glucose and it is a multi-steps reaction. There are two distinct phages in the pathway. The first is Oxidative phase, in which NADPH is generated . The second is the Non-oxidative synthesis of 5-carbon sugars.

4. Oxidative phase Irreversible phase. Dehydrogenation of glucose 6- phosphate to 6- phosphogluconate catalyzed by glucose 6-phosphate dehydrogenase. Followed by hydrolysis of 6-phosphoglucolactone catalyzed by 6- phospho- glucnate dehydrogenase. Decarboxylation follows with the formation of the keopentose ribulose 5-phosphate. both this step requires NADP + as hydrogen acceptor.

5. Non-oxidative phase

6. Significance of Pentose phosphate pathway The Pentose phosphate pathway is fundamentally different from glycolytic pathway because no ATP is generated in the PP pathway , whereas it is a major product of glycolysis . NADPH from the PP pathway is required for many reductive biosynthetic processes , detoxifcation processes, oxidative defenses and ROS (Reactive oxygen species) generation (through the activity of NADPH oxidases). PPP is required for synthesis of sugar in the Calvin cycle. Production of ribose 5- phosphate (R5P) used in the synthesis of nucleotides and nucleic acids. Production of erythrose 4-phosphate (E4P) used in the synthesis of aromatic Amino acids tryosine ,phenylalanine and tryptophan.

7. SUMMARY The Pentose phosphate pathway is a central metabolic pathway that plays essential roles in providing NADPH for biosynthetic processes, generating ribose 5-phosphate for nucleotide synthesis , maintaining redox balance and interconnecting with other metabolic pathways. Overall , PPP is a critical metabolic pathway that contributes to various cellular functions including antioxidant defense , nucleotide synthesis and energy production .

8. REFERENCE Life science by Pathfinder edition. https:/www.wikipedia.org.com https:/www.slideshare.net https:/www.khanacademy.org

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