pH meter Use, Calibration and application

Instrumentation
pH meter use, Application and calibration

1. F.Y.B.Sc ZOOLOGY SEMESTER I PAPER II: UNIT 3 INSTRUMENTATION Presentation by : Veena

2. CONTENTS MICROSCOPY COLORIMETRY pH CENTRIFUGE CHROMATOGRAPHY ELECTROPHORESIS INTRODUCTION, PRINCIPLE (how it works) APPLICATIONS

3. pH meter

4. Potentiometric pH meter

5. APPLICATIONS Use to estimate hydrogen ion concentration Understanding nature of sample (Acidic/Basic/Neutral) Knowing exact pH of sample for manipulating chemical reactions for synthesis of certain molecules Acid based titrations (endpoint graphically determined) Pharmaceutical industry (drug needs to be of sp. pH) Medical science (diagnosis burning throat/tooth decay) Dairy industry (cheese quality and other products) Agricultural (soil pH : crop cultivation, good yield) Aquaculture and water consumption

6. What is pH ? And why there was need of inventing the term Enzymes (reactions in body) Hydrogen ion concentration Danish naturalist ; Soren Peter Lauritz sorenson 1909 ; pH scale

7. pH scale by Danish biochemist : SOREN PETER LAURITZ SORENSON 1909

8. Weak acid & Strong acid Weak base and Strong base Buffer : A solution that resists the change in pH Home work: examples of WA,SA, SB,SA Examples of natural buffers Few must know terminologies/concepts

9. THANK YOU See you all in next class