Pharmaceutical Microbiology Presentation__4.pptx

Culture media factors affecting growth of bacteria
Bacterial growth curve

1. Microbiology Bacteriological Culture media Physical parameters for growth Growth curve Of bacteria Ms. Ayodhya Pardhe ( M Pharmacy) Sudhakar Rao Naik institute of pharmacy Pusad

2. Contents Culture media Ingredients used in culture media Classification of culture media Physical parameters for growth of bacteria Growth curve of bacteria

3. Long chain polysaccharides obtained from seaweed algae Is mixture of two polysaccharides such as aggarose (70%) and agaropectin (30%) Properties of agar: Acts as good solidifying agent It has no nutritional value in media Stable at different temperatures used for microorganisms Melt at 95 to 98 degree Celsius and remains liquid upto 40 to 42 degree Celsius Easily available and economical

4. Water Tap, pure distilled water may be used for preparation of culture media by dissolving various organic and inorganic compounds All enzymatically controlled chemical reaction occurs within cell in presence of water Copper distilled water cannot be used for preparation of culture media because copper inhibits bacterial growth Essential for growth of all microorganisms

5. Components Quantity Functions or use Yeast/meat/beef extracts 10gm Source of carbohydrates, amino acids and other growth factors Peptone 10gm Source of amino acids, N, S,P and growth factors Nacl 5gm Electrolytes Agar 20gm Solidifying agent Distilled water To make- 1000 ml Vehicle

6. Classification of culture media Depending on physical state: Solid media Semisolid media Liquid media Depending on oxygen: Aerobic media e.g. MacConkeys broth Anaerobic media e.g. Robertson s cooked meat medium Depending on chemical composition Simple or basal media Synthetic media No synthetic media or complex media

7. Depending on functional types: Enriched media Enrichment media Selective media Indicator media Differential media Sugar media Transport media Assay media Storage media

8. Indicator media These media contain an indicator which changes colour when a bacterial species grows in them E.g. Wilson and Blair medium Salmonella typhi reduces sulphite to sulphide in presence of glucose and the colonies have black metallic shine

9. Enrichment media When specific substance is added in a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria such media called enrichment media e.g. Tetrathionate broth, selenite F broth

10. Sugar media In sugar medium, 1% sugar is added to peptone water with a suitable indicator Durhams tube (small tube) is kept inverted in the tube containing this medium to detect gas production

11. Assay media: These media have specific composition and are used for the assay of antibiotics, amino acids and vitamins Storage media: This media help in. Preservation and storage of bacteria for long period e.g. Nutrient agar slots, blood agar slants etc.

12. Bacterial growth curve

13. Thank you!