Phytochemical Analysis on MENTHA and BACOPA Dissertation PPT

Dissertation PPT on Phytochemical Analysis on MENTHA and BACOPA

1. Presentation on
PhytochemicalAnalysis on MENTHA and BACOPA
BY
GROUP –B
M.Sc. Botany (2021-23)
Under the Guidance of
Dr. KalpanaPrasad
Head of University Department of Botany
BinodBihariMahtoKoyalanchalUniversity , Dhanbad

2. PytochemicalAnalysis Group
Our group has done Phytochemical
analysis on Menthaand Bacopa.
Phytochemicalsare naturally
present in the plants and show
biologically significance by playing
an essential role to the plants to
defend themselves against various
pathogenic microbes by showing
the antimicrobial activity by
inhibition or killing mechanism.

3. INTRODUCTION
ETHNOMEDICINAL PLANTS
The dependency of human beings on plants is an old
relationship which described as “ETHNOBOTANY ”.
Ethnobotanydeals with various aspects in which one of
the most popular and common aspects is the study and
use of ethonomedicinalplants. Ethnomedicinalplants
involves the study of indigenous beliefs, concept,
knowledge and practices among the ethnic groups of
tribal and rural people for preventing , curing and
treating jaundice.

4. MENTHA
KINGDOM : PLANTAE PLANTS
PHYLUM : MAGNOLIOPH
CLASS: MAGNOLIOPSIDA
ORDER: LAMIALES
FAMILY: LAMIACEAE
GENUS: MENTHA
SPECIES : PIPERITA
The species is one of the oldest known medicinal plants. Mints are
aromatic, almost exclusively perennial herbs. They have wide spreads
underground and overgroundstolonsand erect , square, branched, stems.
Leaves being the most important part from which oil is extracted , the
anatomical characters are relevant. All mint thrives near pools of water,
lakes, rivers, and cool moist spots in partial shades. Peppermint oil is
composed primarily of Menthol (37.4%), Methyl acetate (17.4%) and
Menthone(12.7%).

5. BACOPA
KINGDOME : PLANTAE
CLADE: ANGIOSPERM
ORDER: LAMIALES
FAMILY: PLANTAGINACEAE
GENUS: BACOPA
SPECIES: B. monnieri
Bacopamonnieriis a perennial , creeping herb native to the wetlands of
southern and eastern India, Australia , Europe and North and South
America.Bacopamonnieriis a non-aromatic herb. Theleavesof this plant
are succulent, oblong, and 4–6mm (0.16–0.24in) thick. Leaves
areoblanceolateand are arranged oppositely on the stem. Theflowersare
small,actinomorphicand white, with four to five petals. It can even grow
in slightlybrackishconditions. Propagation is often achieved through
cuttings.

6. OBJECTIVE
PhytochemicalAnalysis is intended to screen , identify , extract
and isolate the phytoconstituentsto evaluate the therapeutic
plant and to develop phytochemicalstandards for the medicinal
plant materials for quality control.
Themainobjectivesare:-
•TofindwhatisactingasMedicinalplantextract.
•Tostandardizethemedicinalplantextract.
•Toascertainpossiblemedicinalactions.

7. MATERIAL AND METHODS
Plant Material and Sample Preparation : -plant extract of Menthaand
Bacopaare collected. They were rinsed with tap water followed by
distilled water to remove the dirt on the surface.Barkswere removed
from stems and cut into small pieces. They were then air dried for 2 days
and then freeze dried until a constant mass was Obtained. Dried samples
were ground into fine powder and Kept in desiccators until extracted. The
extraction was carried Out in a soxhletapparatus for 10 h using absolute
methanol.Thesolvent was then evaporated using rotary evaporator and
the crude extracts were kept in desiccators.

8. Chemical Reagents
Water, ferric chloride, acidified alcohol, ammonia, chloroform, acetic
acid, Mayer’s reagent, Dragendroff’sreagent, sulfuric acid, sodium
hydroxide, Fehling’s solution, Molisch’sreagent, hydrochloric acid, zinc
dust, magnesium turnings, biuretreagent, Folin–Ciocalteureagent,
sodium carbonate, gallicacid solution, acetone and isopropyl alcohol
(IPA), ethanol, methanol, aluminiumtrichloride, potassium acetate,
quercetinsolution, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), dimethyl
sulfoxide, glucose, glimepiride, diclofenacsodium, etc.

9. Methodology
Qualitative screening:-
The qualitativephytochemicalscreening of the extracts was performed to identify them a in
groups of chemical constituents
(glycosides,alkaloids,tannins,saponins,terpenoids,carbohydrates,cardiacglycosides,anthraquinone
s glycosides,flavonoids,andphenols) present in the extracts using the color reactions.
Our group has done these following test :-
Test for tannins
Testfor alkanoids
Test for glycoside
Testfor carbohydrates ( Molisch‘s test )
Test for saponins
Test for cardiac glycosides
Testfor flavonoids
Testfor terpenoids( Salkowski’stest )
Test for protein
Test for phenol

10. TEST FOR TANNINS
TEST FOR ALKANOIDS
TEST FOR
CARBOHYDRATE
TEST FOR PROTEIN

11. TEST FOR CARDIAC
GLYCOSIDES
TEST FOR FLAVONOIDS
TEST FOR SAPONINS

14. References
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