Plant tissue culture- In-vitro Rooting.ppt

In vitro means production in a test tube or other similar vessel where culture conditions and medium are controlled for optimum growth during tissue culture.
It is a critical step in plant tissue culture where roots are induced and developed from plant explants in a controlled, sterile environment.
Slide include factors affecting In-vitro Rooting, steps involved, stages and In vitro rooting of the two genotypes of Argania Spinosa in different culture media.

1. In-vitro rooting
Paper: Plant tissue culture
BY: LAXMI CHOUDHARY
DEPARTMENT OF BOTANY JAI
NARAIN VYAS UNIVERSITY,JODHPUR

2. Content
• Introduction
•Factors affecting
•Steps involved
•Stages
•Importance in vitro rooting
•rooting of the two genotypes of Argania Spinosa
•References

3. What In-vitro rooting means?
● In vitro means production in a
test tube or other similar vessel
where culture conditions and
medium are controlled for
optimum growth during tissue
culture.
● It is a critical step in plant tissue
culture where roots are induced
and developed from plant
explants in a controlled, sterile
environment.

4. Factors affecting in vitro rooting
• Plant growth regulators:
Auxin- help initiate rooting.
Others- IBA, NAA are other derviates of auxin.
• Nutrients:
Zinc, nitrogen and phosphorus are necessaryto induce roots
• Environment conditions:
Low light or darkness, slightly low temperature(20°C) and medium humidity
are factors required for root induction.
• Vitamins:
Vitamin D3 stimulates rooting of Phaseolus vulgaris L.
More riboflavin was added rooting decreased in a linear form until it was
completely inhibited in peach rootstock GF677 (Prunus amygdalus × P. persica
Batsch.)

5. Steps include
• Ex plant selection
•Sterilization
•estabalization in growth media
•Induction of root by hormone treatment
•Incubation
•Root formation
•Acclimation
•Transplantation

6. Stages
Two stage-
1. Root initiation- formation of root primodia at
base of explant
2. Root development- elongation of induced root
and secondary development.

7. Importance of rooting?
• The presence of roots in the plantlets resulted in
higher values of biometric features, such as leaf
area, numbers of leaves and secondary roots, nodal
segments, fresh and dry weights of leaves, when
compared to plantlets without roots.
•Roots well established also resulted in higher levels
of chlorophyll a, chlorophyll b, and total chlorophyll
and a higher photosynthetic rate

8. In vitro rooting of the two genotypes of
Argania Spinosa in different culture media
•(1) indicates genotype “Mejji”, and (2) indicates
genotype “R’zwa”.

9. In presence of putrescine
and indole-3-butyric acid
(IBA) in Murashige and
Skoog (MS) medium
In presence of putrescine and
IBA in MS medium modified by
reducing ammonium nitrate
concentration to 825 mg L−1
(RM medium)
In presence of putrescine
and IBA in MS medium
without ammonium
nitrate (M medium)
In presence of silver nitrate
(AgNO3) (2 mg L−1) and IBA
In presence of AgNO3 (4 mg
L−1) and IBA
In presence of AgNO3 (6 mg
L−1) and IBA

10. In presence of AgNO3 (2
mg L−1), putrescine and IBA
In RM medium containing
AgNO3 (2 mg L−1),
putrescine and IBA
In RM medium containing AgNO3
(2 mg L−1), putrescine, IBA and 1-
naphthalene acetic acid (NAA)
(0.5 mg L−1)
In RM medium containing
AgNO3 (2 mg L−1),
putrescine, IBA and NAA
(1 mg L−1)
In RM medium containing
AgNO3 (2 mg L−1),
putrescine, IBA and NAA (1.5
mg L−1)
In RM medium containing
AgNO3 (2 mg L−1),
putrescine and NAA (1.5 mg
L−1)

11. References
•
https://propg.ifas.ufl.edu/09-tissue-culture/03-ter
ms/08-tcterms-exvitroinvitro.html
•https://www.sciencedirect.com/science/article/pii/
S2214662821000141
•https://www.scirp.org/html/8740.html
•https://www.mdpi.com/2223-7747/10/6/1062

12. Thank you