PLATELET COUNT by Dr. Pandian M .pptx

This ppt for only purpose of study. MBBS, BPTH, and Other medicos can use this

1. PLATELET COUNT Dr. Pandian M Dept. of Physiology DYPMCKOP

2. MATERIALS: MICROSCOPE NEUBAUER’S CHAMBER PLATELET DILUTING FLUID (REES ECKER FLUID) RBC PIPETTE COTTON,SPRIT,LANCET

3. what is the functions of Platelets It serve four main functions in the body : Temporary hemostasis Clotting of blood Phagocytosis of small particles and organisms, and Storage and transport of chemicals

4. Normal values – 1.5 to 4 lakhs / cumm . Average 2.59 lakhs / cumm . Size - Diameter : 2.5 μ (2 to 4 μ) . Life span – 8 to 12 days.

5. REES ECKER FLUID: Composition : Sodium citrate- prevents the coagulation, preserves RBC,provides the necessary low specific gravity Formaline -act as fixative Distilled water -solvent Other fluid can used- 1% ammonium oxalate RBC Diluting fluid we’re using here

6. PROCEDURE: Focus Neubauer’s chamber under low power Under aseptic precaution take a bold prick Suck blood up to 1 mark Followed by platelet diluting fluid up to 101 Mix thoroughly by holding between palms Discard first 2 drops and charge the chamber Count the cells 4 corner square and 1 central square

7. Calculation Volume of small chamber = 1/5 x 1/5 x 1/10 = 1/250 mm 3 Total volume of 5 small chambers = 1/250 x 5 = 1/50 mm 3 Total number of cells = 9 + 6 + 8 + 9 + 8 = 40 1/50 mm 3 of blood contains = 40 cells ∴ 1mm 3 of blood will contain = 40 x 50 = 2000 cells Dilution factor = 1 in 100 ∴ total number of cells = 2000 x 100 =200000 = 2 lakh / mm 3 of blood Result :- The platelet count is 2 lakh /mm 3 of blood.

8. THROMBOCYTOSIS – Increase in the number of platelets above normal count Polycythemia vera , Iron deficiency anemia , Splenectomy , Bone marrow malignancy, After trauma e.g. Surgery, injury, child birth, etc .

9. THROMBOCYTOPENIA - Decrease in the no of platelets below normal count. Bone marrow depression Hypersplenism Viral infections – e.g. dengue fever, it particularly attacks platelets. Drug hypersensitivity and etc.

10. Thrombocythemia - ?? Glanzmann’s Thrombasthenia -????? Inherited hemorrhagic disorder, Caused by structural or functional abnormality of platelets. The platelet count is normal. It is characterized by normal clotting time, normal or prolonged bleeding time but defective clot retraction.