POUR PLATE TECHNIQUE.pptx

Aim, Principle, Materials, Procedure and Results for the Pour plate technique

1. Pour plate technique M.J. AFRA UG Scholar Department of microbiology Thassim beevi abdul kader college for women kilakarai

2. AIM To isolate microorganisms from the sample To get pure culture of microorganisms

3. PRINCIPLE Pour plate is a rapid quantitative isolation method This method is used to isolate bacteria, fungus, and actinomycetes Original sample is diluted several times to thin out the population sufficiently The most diluted sample is mixed with warm agar and poured into petriplates A fter the agar solidifies and forms individual colony The total number of colonies equals to the number of viable microorganisms Colonies were transferred on fresh media for identification

4. MATERIALS REQUIRED Test tubes with 9ml of diluents Cotton Autoclave Hot air oven Water bath Nutrient agar PDA medium Petri plates Pipettes

5. PROCEDURE Prepared nutrient agar and PDA medium and sterilize at 121 o C for 15 minutes Dilute the sample upto 10 -7 Add 1ml of sample from the 10 3 to the petri plate and label them Pour the medium into sample added petriplates Rotate the petri plates clockwise and anticlockwise direction and allow it to solidify Similiarly perform pour plating for other dilution from 10 -4 to 10 6 Incubate all the nutrient agar plates at 37 o C for 24 hours And incubate PDA plates at 25 – 30 o C for 48 hours and record the result

6. ADVANTAGES & DISADAVANTAGES ADVANTAGES DISADVANTAGES Need large volume of sample than other methods Heat sensitive organisms are destroyed with melted agar Allow the growth of the micro aerophiles beneath the surfaces of the medium Some colonies are formed beneath the surface of the agar, these colonies are not satisfactorily used for identification

7. RESULT Individual colonies are noted Calculated the number of bacteria present in the sample