ppt bioteknologi protoplasma fusion between indonesian citrus maxima

ppt bioteknologi

1. Protoplast Fusion between Indonesian Citrus maxima ( Burm .) Merr . and Citrus reticulata L. Review Jurnal Mizuka Audri NIM Putri Novitasari 130820036 Mata Kuliah : Bioteknologi Tanaman Dosen Pengampu : Dr. Reni Indrayanti, M.Si Rizky Priambodo, M.Si

2. 01 PENDAHULUAN 02 METODE 03 HASIL DAN PEMBAHASAN

3. PENDAHULUAN 01

4. PENDAHULUAN Fusi protoplas merupakan salah satu teknologi alternatif untuk menghasilkan tanaman triploid, sekaligus kombinasi sel somatik dari berbagai kultivar , spesies atau genus menghasilkan kombinasi genetik baru ( Wulandari 2018) Jenis penggabungan protoplas pada proses fusi yang mungkin terjadi adalah homofusi antara protoplas daun atau antar protoplas kalus , heterofusi antara protoplas daun dan protoplas kalus , multifusi antara protoplas daun dan kalus (Hennig et al ., 2015; Husni, 2010) Fusi protoplas telah digunakan untuk melengkapi breeding konvensional yang menghasilkan tanaman triploid tidak berbiji dan meningkatka kualitas buah . Selain itu fusi protoplas bisa digunakan untuk transfer gen dalam pengembangan kultivar baru untuk mengatasi beberapa permasalahan termasuk ketidakcocokan seksual , embrio nucelar ( poliembrioni ), dan kemandulan induk (Guo et al ., 2013)

5. PENDAHULUAN Hibridisasi somatic antara pummelo dengan jeruk keprok diharapkan menghasilkan buah jeruk bali berukuran sedang , kulit lebih tipis, tahan terhadap CTV (Citrus Tristeza Virus) dan tanaman tetraploid Jeruk keprok dipilih sebagai tanaman induk karena mudah dikupas , daging buah yang bagus , dan mampu membentuk embriogenik somatic. Jeruk keprok memiliki ciri khas warna daging jingga , rasa segar dan manis. Penelitian ini bertujuan untuk mengetahui isolasi protoplas , fusi protoplas antara Jeruk bali ' Nambangan ’ (Citrus maxima) dan Jeruk keprok ' Garut ' (C. reticulata), serta kultur protoplasnya

6. METODE 02

7. Fusi protoplas antara Citrus maxima ( Burm .) Indonesia Merr . dan Citrus reticulata L. diamati secara detail di Laboratorium Kultur Sel dan Jaringan Tumbuhan , Pusat Penelitian Bioteknologi , Lembaga Ilmu Pengetahuan Indonesia selama bulan Januari-Agustus 2015 Waktu dan Tempat Penelitian :

8. 01 02 03 Isolasi protoplas dilakukan dengan metode yang dikembangkan oleh Grosser & Gmitter (1990). Untuk mengisolasi protoplas , daun jeruk bali yang ditanam secara in vitro dipotong menjadi strip tipis (1-2 mm) secara horizontal di sepanjang pelepah daun , dan gumpalan kalus embriogenik dipotong kecil-kecil Metode Isolasi Protoplas Kemudian diinkubasi dalam larutan enzim dengan volume total 12 ml yang diencerkan dengan media BH3 menggunakan labu Erlenmeyer 100 ml. Setelah itu diinkubasi Kembali selama 16 jam pada suhu 28 ° C dalam gelap pada pengocok putar dengan kecepatan 50 rpm. Protoplas dimurnikan dengan saringan nilon (50- 100 µm mesh). Pengendapan filtrat dilakukan dengan cara sentrifugasi pada kecepatan 800 rpm selama 8 menit . Pelet yang mengandung protoplas disuspensikan dalam media BH3. Kemudia diamati di bawah mikroskop terbalik (Leica, DMIL LED) dengan perbesaran 400. Analisis statistic diameter protoplas dilakukan dengan (ANOVA).

9. Fusi protoplas dilakukan dengan menggunakan metode PEG (Grosser & Gmitter , 1990). Larutan PEG (BM = 600) 40%, ditambah glukosa 0,3 M, dan CaCl2 66 mM pada pH = 6. Larutan elusi A terdiri dari glukosa 0,4 M, CaCl2 66 mM , DMSO 10% pada pH = 6, dan larutan B terdiri dari glisin 0,3 M pada pH 10,5. Larutan A dicampur dengan B sebelum ditambahkan ke piring fusion. dengan perbandingan 9:1 (v/v). Protoplas dari kalus embriogenik dan mesofil daun dicampur dengan volume yang sama (1:1). Fusi protoplas diinduksi dalam plastik steril Cawan petri (60 x 15 mm). Ke dalam masing-masing cawan fusi , ditambahkan 2 tetes campuran protoplas , dan 2 tetes PEG 40%. Setelah 8-10 menit , 2 tetes larutan A+B ditambahkan ke dalam setiap cawan fusi di pinggiran campuran protoplas . Setelah 12-15 menit ditambahkan 12 tetes medium BH3 0,6 M. Setelah 5 menit semua cairan dikeluarkan perlahan dari cawan dengan pipet Pasteur dan diganti dengan 12-15 tetes media BH3 0,6 M. Prosedur pencucian ini diulangi dua kali. Terakhir , ditambahkan media BH3 0,6 M 1,5-2,0 ml, dan kultur protoplas ditempatkan ke dalam cawan Petri plastik berukuran 60 x 15 mm yang tertutup rapat , kemudian diinkubasi dalam gelap pada suhu 25-27 °C Metode Fusi Protoplas

10. Jenis fusi protoplas ( homofusi , heterofusi dan multifusi ) diamati menggunakan mikroskop terbalik (Leica DMIL LED) pada perbesaran 400, setelah penambahan PEG selama 8-10 menit . Protoplas yang menyatu diidentifikasi dengan adanya kloroplas dari daun jeruk bali “ Nambangan ” dan butiran pati dari kalus embriogenik keprok ' Garut '. Metode Fusi Protoplas

11. 01 03 Medium untuk kultur protoplas adalah medium BH3 cair dengan 2 konsentrasi yaitu 0,6 M dan 0,4 M (Grosser & Gmitter , 1990). Media padat untuk pertumbuhan koloni sel terdiri dari vitamin MW dengan penambahan 3 mg L-1 BAP ( Karyanti , 2013; Merigo , 2011) Kultur Protoplas yang Menyatu Fase pertumbuhan protoplas diamati menggunakan mikroskop terbalik (Leica DMIL LED). Koloni sel terbentuk pada minggu keempat ; Pembentukan kalus mikro diinduksi dengan menurunkan osmolaritas medium dengan menambahkan 10-12 tetes 0,4 M BH3. Parameter pertumbuhan awal dihitung berdasarkan jumlah koloni sel dan diameter koloni sel yang terbentuk pada 10 cawan Petri pada minggu keempat menggunakan mikroskop stereo (Leica EZ4HD) dengan 8 perbesaran . Setiap petri diwakili oleh bidang pandang tunggal dengan lebar 142,45 mm2. Uji t dilakukan untuk analisis data setiap paramete Protoplas yang menyatu dan protoplas dari kalus embriogenik keprok ' Garut ' dikultur pada cawan Petri (60 x 15mm) dalam 8-12 tetes media 0,6 M BH3. Media yang sama ditambahkan setiap minggu untuk menjaga kelembapan . Kultur protoplas diinkubasi dalam ruangan gelap pada suhu 25-27°C selama 4-6 minggu 02

12. Isolasi Protoplas Mercury is the closest planet to the Sun Features o f Goyang 01 Jupiter It’s a gas giant and the biggest planet in the Solar System 02

13. This can be the part of the presentation where you can introduce yourself, write your email… Whoa!

14. —Someone Famous “This is a quote, words full of wisdom that someone important said and can make the reader get inspired.”

15. Competitors 01 You can describe the topic of the section here

16. Do you know what helps you make your point clear? Lists like this one: They’re simple You can organize your ideas clearly You’ll never forget to buy milk! And the most important thing: the audience won’t miss the point of your presentation What is this festival?

17. Mercury Mercury is the closest planet to the Sun Features o f Goyang 01 Jupiter It’s a gas giant and the biggest planet in the Solar System 02

18. Mercury Mercury is the closest planet to the Sun Taking care of flowers Mars Despite being red, Mars is actually a very cold place Venus Venus has a beautiful name and is the second planet

19. A picture always reinforces the concept Images reveal large amounts of data, so remember: use an image instead of a long text. Your audience will appreciate that

20. A p icture i s w orth a thousand words

21. Awesome Words

22. Countries with more participants To modify this graph, click on it, follow the link, change the data and paste the new graph here Netherlands Jupiter is the biggest planet USA Neptune is the farthest planet Korea Mercury is the closest planet China Venus is the second planet 34% 23% 26% 18%

23. Countries 02 You can describe the topic of the section here

24. Mercury It’s the closest planet to the Sun Maybe you need to divide the content Venus Venus is the second planet to the Sun Mars Mars is actually a very cold place Jupiter Jupiter is the biggest planet Saturn It’s a gas giant and has several rings Neptune Neptune is the farthest planet

25. An infographic always works well Jupiter 80% Mercury 10% Venus 40% Mars 50% It is a gas giant It’s the closest It is the second It’s a cold place

26. Schedule of April Mon Tue Wed Thu Fri Sat Sun Neptune is the farthest 29 30 31 01 02 03 04 It is the closest planet 05 06 07 08 09 10 11 Venus has a nice name 12 13 14 15 16 17 18 Earth is the third planet 19 20 21 22 23 24 25 Mars is a cold place 26 27 28 29 30

27. Types of plants according to the region Jupiter Jupiter is a huge gas giant Mars Mars is actually a cold place Mercury Mercury is the closest planet Neptune Neptune is the farthest planet

28. 01 02 03 04 Venus has a beautiful name Venus Mercury is the closest to the Sun Mercury Despite being red, Mars is a cold Mars Jupiter is the biggest planet Jupiter Our history

29. Our diagram about the festival Mercury Saturn Ceres Sun Jupiter Saturn Earth Venus Mars Pluto

30. Jupiter is a gas giant and the biggest planet in the Solar System. It's the fourth-brightest object in the night sky The competition

31. Types of flowers Neptune Neptune is the farthest planet from the Sun 70% Saturn Saturn is a gas giant and has several rings 90% Jupiter Jupiter is a gas giant and the biggest planet 50%

32. You can replace the image on the screen with your own work. Right-click on it and then choose "Replace image" (in Google Slides) or "Change Picture" (in PPT) so you can add yours Multimedia

33. Jupiter Jupiter is a gas giant and the biggest planet Saturn Saturn is a gas giant and has several rings Mercury Mercury is the closest planet to the Sun Neptune Neptune is the farthest planet from the Sun Parts of a flower

34. Saturn is a gas giant and has several rings. It's composed mostly of hydrogen and helium 24th of April

35. 150,000 Big numbers catch your audience’s attention

36. 1,000 It’s the closest planet to the Sun 2,000 Venus is the second planet from the Sun 15,000 It’s composed of hydrogen and helium 5,000 It’s the farthest planet from the Sun

37. You can use five columns Mercury It’s the closest planet to the Sun Venus Venus is the second planet from the Sun Mars Mars is actually a very cold place Jupiter It’s the biggest planet to the Sun Saturn It’s a gas giant and has several rings

38. Sarah Patterson Here you can talk a bit about this person Our team Timmy Jimmy Here you can talk a bit about this person

39. Poppies Mars Mars is actually a cold place Jupiter It was named after a Roman god Mercury Mercury is the smallest planet Venus Venus has a beautiful name

40. Desktop software You can replace the image on the screen with your own work. Right-click on it and then choose "Replace image" so you can add yours

41. Tablet app You can replace the image on the screen with your own work. Right-click on it and then choose "Replace image" so you can add yours

42. Mobile app You can replace the image on the screen with your own work. Right-click on it and then choose "Replace image" so you can add yours

43. Thanks Do you have any questions? [email protected] [phone] yourcompany.com Please keep this slide for attribution

44. Alternative resources

45. House plants icon pack

46. Resources Vectors F lat spring wallpaper with empty space Hand drawn spring background Han d drawn spring flowers background Creative drawn s pring background Floral element collection Flowe r leaf collection Collection grass border silhouettes Hand drawn spring floral frame Creative welcome landing page with gradient landscape Icons H ouse plants Photos Young woman appreciating the nature surrounding her Front view woman with flowers outdoors Beautiful asian woman posing holding yellow flowers with perfect skin

47. Instructions for use ( free users) In order to use this template, you must credit S lidesgo by keeping the Thanks slide. You are allowed to: Modify this template. Use it for both personal and commercial purposes. You are not allowed to: Sublicense, sell or rent any of Slidesgo Content (or a modified version of Slidesgo Content). Distribute this Slidesgo Template (or a modified version of this Slidesgo Template) or include it in a database or in any other product or service that offers downloadable images, icons or presentations that may be subject to distribution or resale. Use any of the elements that are part of this Slidesgo Template in an isolated and separated way from this Template. Delete the “Thanks” or “Credits” slide. Register any of the elements that are part of this template as a trademark or logo, or register it as a work in an intellectual property registry or similar. For more information about editing slides, please read our FAQs or visit Slidesgo School: https://slidesgo.com/faqs and https://slidesgo.com/slidesgo-school

48. Fonts & colors used This presentation has been made using the following fonts: Patrick Hand ( https://fonts.google.com/specimen/Patrick+Hand ) Nanum Gothic ( https://fonts.google.com/specimen/Nanum+Gothic ) #fad49f #ffa56c #594abf #ffec81 #9080ff #89d7c3 #4cc2a3 #3e9e84 # ffeebe #3a4258

49. Storyset Create your Story with our illustrated concepts. Choose the style you like the most, edit its colors, pick the background and layers you want to show and bring them to life with the animator panel! It will boost your presentation. Check out How it Works. Pana Amico Bro Rafiki Cuate

50. You can easily resize these resources without losing quality. To change the color , just ungroup the resource and click on the object you want to change. Then, click on the paint bucket and select the color you want. Group the resource again when you’re done. You can also look for more infographics on Slidesgo. Use our editable graphic resources...

51. JANUARY FEBRUARY MARCH APRIL MAY JUNE PHASE 1 PHASE 2 Task 1 Task 2 Task 1 Task 2 JANUARY FEBRUARY MARCH APRIL PHASE 1 Task 1 Task 2

52. ...and our sets of editable icons You can resize these icons without losing quality. You can change the stroke and fill color ; just select the icon and click on the paint bucket/pen . In Google Slides, you can also use Flaticon’s extension , allowing you to customize and add even more icons.

53. Educational I cons Medical Icons

54. Business I cons Teamwork Icons

55. Help & Support I cons Avatar Icons

56. Creative Process I cons Performing Arts Icons

57. Nature Icons

58. SEO & Marketing Icons