Ppt Presentation on sterilization methods

In this includes the introduction, importance and various methods of sterilization

1. Microbiology Sterilization methods Ms. Ayodhya Pardhe (M Pharmacy) Sudhakar Rao Naik institute of pharmacy Pusad

2. Contents Introduction Importance of sterilization Sterilization methods Physical methods Chemical methods Mechanical methods Radiation methods

3. Importance of sterilization To prevent contamination in sterile products To prevent decomposition and spoilage of food products To prevent transmission of pathogenic microorganisms which responsible for causing diseases. To prevent contamination of unwanted microbes in pure culture media To prevent unwanted microbes contamination in antibiotics, enzymes, vitamins etc To prevent contamination in aseptic areas which are used for the preparation of sterile dosage form and sterility testing

4. Methods of sterilization Physical methods Dry heat sterilization eg . Hot air oven, red heat, direct flame, incineration Moist heat sterilization eg . Autoclave, pasturization , tyndallization Chemical methods Gaseous sterilization eg . Formaldehyde, ethylene oxide etc By using disinfectants eg . Cresol, phenol etc

5. 3. Mechanical methods ( filtration methods) Asbestos filter (Seitz filter) Filter candles (ceramic or Berkefeld filter) Membrane filter Sintered glass filter ( morton filter) Radiation methods Use of ultra violet rays: UV rays Ionizing radiation: X-rays, gamma rays, beta rays

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