PRESENTASI ISPO bIODEGRADABLE sURFACTANT [Autosaved].pptx

lIMBAH MINYK JELANTAH MENJADI SABUN

1. Effectiveness of Azospirillum and Pseudomonas Bacteria on Biodegradable Surfactant ( BISUK) ENVIRONMENT Lintang Perwitasari Tasya Regita P. SMA Kesatuan Bangsa

2. What is BISUK?

3. Keyword 3 Waste cooking oil Bioremediation Detergent

4. BACKGROUND 4 F ood agroindustry increase about 1 8 % every year in various regions (Badan Pusat Statistik) L aundry services need a high amount of detergent.

5. PROBLEM FORMULATION Can waste cooking oil be used for making detergent ? What is the most effective bacteria for detergent bioremediation between Azospirillum sp. and Pseudomonas aeruginosa ? What is the best composition to make a detergent from waste cooking oil ? 5

6. Reduce the waste of cooking oil 6 ADVANTAGES Produce a biodegradable detergent

7. 7

8. Why did we cho o se Pseudomonas and Azospirillum sp. for bioremediation?

9. Methodology 9

10. M ethodology of Methyl Ester Production Collecting the Materials 10 Purification of waste cooking oil Synthesise of Methyl Este r

11. Methodology Biodegradable Test 11

12. M ethodology of Producing Detergent Sul f onation of Methyl Ester 12 Test the product Production of detergent

13. R esult Characteristic of BISUK OD Count Using UV-VIS Spektrometer Organoleptic Test 13

14. Table OD Count 14 Note : Ratio = methyl ester and bacteria A = Methyl ester NaOH 15 % B = Methyl ester NaOH 20 % C = LAS (conventional surfactant)   No Product Ratio Absorption Pseudomonas Bacteria Azospirillum Bacteria 1 A 1:2 1,817 0,013 1:1 0,002 0,058 2 B 1:2 1,101 0,319 1:1 0,281 0,963 3 C 1:2 0,191 0,015 1:1 0,121 0,008

15. Table Characteristic of BISUK 15 Parameter Characteristic SNI State Shape Smell Color   Homogeneous fluids Rose , W hite   pH 9 9-10 Foam power (V60/V0) 1,8 3,2 Free fatty acid levels 6,2-8,6 0,3

16. G raph Organoleptic Test 16 A = NaOH 20% with ratio of MES and water is 1:12   B = NaOH 20 % with ratio of MES and water is 1:19   C = NaOH 15 % with ratio of MES and water is 1:12   D = NaOH 15% with ratio of MES and water is 1:19  

17. C onclusion The waste of cooking oil can be converted into a biodegradable surfactant Based on the OD calculation the most effective bacteria is Pseudomonas. The best product is Methyl ester sulfonate use 15 % NaOH with ratio of MES and water is 1:19. 17

18. SUGGESTIONS Further research on the best formula for BISUK’s viscosity and washing power. Measurement of methyl ester after bioremediation using GC-MS. Socialization to the wider community. 18

19. 19 THANK YOU Sincerely, Lintang Perwitasari | Tasya Regita Pramesti