Properties of plant that can be used for the PTC

Plant Properties

1. Plant Biotechnology 23BBT3CA PLASTICITY AND TOTIPOTENCY 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

2. PTC Environment Unit I Course Outcome: To know about environmental conditions and media required for PTC 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

3. Requiremen t s Tissue culture has several critical requirements: Appropriate tissue A suitable growth medium Aseptic (sterile) conditions Growth regulators Frequent subculturing 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

4. Basic Tissue Culture Procedures 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

5. Environmental Conditions There are three important aspects in vitro culture Nutrient medium Aseptic condition Aeration of the tissue 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

6. Nutrient Medium Medium depends upon the type of plant tissue or cell used for culture Generally nutrient consist of inorganic salts (both micro & macro elements) a carbon source (usually sucrose) Vitamins (eg. nicotinic acid, thiamine, pyridoxine Amino acids (eg. arginine) Growth regulators (eg. auxins) An optimum pH (5.7) is also vary important 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

7. Culture media Explants are then usually placed on the surface of a solid culture medium, but are sometimes placed directly into a liquid medium, when cell suspension cultures are desired. Culture media are generally composed of inorganic salts plus a few organic nutrients, vitamins and plant hormones. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

8. As cultures grow, pieces are typically sliced off and transferred to new media ( subcultured ) to allow for growth or to alter the morphology of the culture. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

9. Aseptic Condition Nutrient medium contains sugar which increases growth of microbes These microbes compete with growing tissue and finally kill it. It is important to maintain aseptic condition. Sterilization is very important to stop the growth of microbes . 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

10. Sterilization Sterilization Methods Used in Tissue Culture Laboratory - All the materials, e.g., vessels, instruments, medium, plant material, etc., used in culture work must be free from microbes 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

11. STERILIZATION TECHNIQUES sterilization is achieved by one of the following approaches: (i) dry heat treatment (ii) flame sterilization (iii) autoclaving (iv) filter sterilization (v) wiping with 70% ethanol (vi) surface sterilization. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

12. Aeration of the Tissue Proper aeration of the cultured tissue is also an important aspect of culture technique. It is achieved by occasionally stirring the medium by stirring or by automatic shaker . 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

13. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

14. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

15. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

16. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

17. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

18. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

19. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

20. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

21. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

22. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

23. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

24. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

25. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02

26. 23BBT3CA - Plant BIotechnology - Dr S Aravindh Unit I - Lecture 02