Pyruvate Dehydrogenase complex and its significance

A detailed analysis on Pyruvate Dehydrogenase Complex

1. Pyruvate Dehydrogenase complex Supervised by Mr. Tridip Boruah Assistant professor m.C. college, barpeta Submitted by Monika Das M.Sc 2 nd semester (Botany) MC College, Barpeta

2. Pruvate Dehydrogenase complex (pyruvate oxidation) The pyruvate dehydrogenase complex catalyzes the irreversible oxidative decarboxylation of pyruvate to form acetyl-CoA. This reaction links glycolysis and the citric acid cycle in every cell containing mitochondria.

3. Conversion of pyruvate to Acetly Coa

4. Pdh complex Pyruvate Dehydrogenase complex is an assembly of three individual enzymes: Pyruvate Dehydrogenase ( E1), Dihydrolipoyl transacetylases (E2) and Dihydrolipoyl dehydrogenase (E3).

5. TPP : Thiamine phyrophosphate L : Lipoyl group CoA : Co enzyme A Pyruvate Dehydrogenase complex Decarboxylation and oxidation of pyruvate Catalyses transfer of acetyl Group to CoA Reoxidizes dihydrolipoamide

6. Regulation by end product inhibition

7. Conclusion In pyruvate Dehydrogenase complex there are three multienzyme complex pyruvate Dehydrogenase complex (E1), Dihydrolipoyl transacetylase (E2) and Dihydrolipoyl Dehydrogenase (E3) which increases the efficiency of the reaction. Also five coenzymes are involved thiamine phyrophosphate (TPP), lipoic acid, fad, nad + and co enzyme a acting association with E1,E2 and E3 in pdh complex. Acetly – Coa and nadh , both are the end product of pdh Complex. Again , 2.5 atp is formed during the reaction.

8. Reference Life sciences by pathfinder ( vol 1) Fundamentals of biochemistry by Dr. J.l . jain , Dr. S. Jain and nitin jain

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