Magnify the unseen world Intricate details unfurled Cells and Creatures, big and small, Revealed beneath the lens, we call Observing life in its tiny scope Scientific wonders, giving hope Concealed mysteries, now clear Optical marvels bring them near Patterns, structures, come alive Exploring realms that thrive
The Microscope What I know What I wonder What I learned
SCIENCE 7 second QUARTER- lc 1 Matatag curriculum Exploring the Compound Microscope
Exploring the Compound Microscope CONTENT Familiarity and proper use of a compound microscope are essential to observe cells. LEARNING STANDARD Identify the parts and functions, and demonstrate proper handling and storing of a compound microscope LEARNING COMPETENCY
By the end of the lesson, the 80 percent of the learners will be able to: Identify the main parts of a compound microscope and describe their functions. Demonstrate the proper handling and storing of a compound microscope. Appreciate the compound microscope by answering the reflection of learning. OBJECTIVES
I. Activating Prior Knowledge Short Review Learners will identify the parts of compound microscope. I. Activating Prior Knowledge Eyepiece (Ocular Lens): Provides the final magnification of the image. Usually 10x magnification.
I. Activating Prior Knowledge Short Review Learners will identify the parts of compound microscope. I. Activating Prior Knowledge Objective Lenses: Provide the initial magnification of the specimen. Typically come in 4x, 10x, 40x, and 100x magnifications
I. Activating Prior Knowledge Short Review Learners will identify the parts of compound microscope. I. Activating Prior Knowledge Revolving Nosepiece: Holds the different objective lenses and allows the user to switch between them.
I. Activating Prior Knowledge Short Review Learners will identify the parts of compound microscope. I. Activating Prior Knowledge Stage: Platform where the specimen slide is placed. Usually has clips to hold the slide in place.
I. Activating Prior Knowledge Short Review Learners will identify the parts of compound microscope. I. Activating Prior Knowledge Diaphragm: Controls the amount of light reaching the specimen. Adjusts the size of the opening to regulate illumination.
I. Activating Prior Knowledge Short Review Learners will identify the parts of compound microscope. I. Activating Prior Knowledge Coarse Adjustment Knob: Moves the stage up and down to focus the image roughly.
I. Activating Prior Knowledge Short Review Learners will identify the parts of compound microscope. I. Activating Prior Knowledge Fine Adjustment Knob: Provides precise focusing of the image.
Identify the main parts of a compound microscope and describe their functions. Demonstrate the proper handling and storing of a compound microscope. Appreciate the compound microscope by answering the reflection of learning. B. Establishing Purpose of the Lesson 1. LESSON PURPOSE
B. Establishing Purpose of the Lesson 2. Unlocking Content Vocabulary: Match Type Activity Resolution Illumination Stage is the measure of the sharpness and clarity of an image produced by the microscope. is the process of providing adequate, even, and controlled lighting to the specimen under observation in order to obtain a clear, high-contrast image . is a flat, horizontal platform located below the objective lens of the microscope, where the specimen slide is placed and secured for observation. Magnification is the process of enlarging the appearance of an object or specimen when viewed through an optical instrument.
C. Developing and Deepening Understanding Microscope is an optical instrument used to observe small objects or details that are not visible to the naked eye. It uses lenses to magnify the image of the object being examined, allowing the user to see it in greater detail. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Eyepiece (Ocular Lens): The lens at the top of the microscope that you look through, usually with a magnification of 10x or 15x. The eyepiece, also called the ocular lens, is the lens closest to the user's eye when looking through a microscope. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Arm: The part of the microscope that connects the base to the head and the eyepiece tube. It is used to carry the microscope. The arm is typically made of a sturdy metal material to ensure the microscope remains stable and secure during use and transport. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Stage Clips: Metal clips on the stage that hold the slide in place. Stage clips come in different designs, such as spring-loaded clips or screw-down clips. They provide a stable platform to keep the specimen centered and focused as the user examines it through the microscope. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Coarse Adjustment Knob: A larger knob used for focusing the microscope. It moves the stage or the body tube up and down to bring the specimen into general focus. Turning the coarse adjustment knob moves the objective lens a larger distance, allowing for faster focusing. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Fine Adjustment Knob: A smaller knob used for fine-tuning the focus of the specimen after using the coarse adjustment knob. It moves the stage slightly to sharpen the image. The fine adjustment is essential for getting the most detailed, high-resolution observation of the sample under the microscope. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Draw Tube: The tube that connects the eyepiece to the microscope body. The draw tube is the cylindrical tube that connects the eyepiece (ocular lens) to the main body or frame of the microscope. This is an important component that enables the user to optimize the performance and viewing experience when using the microscope. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Revolving Nosepiece: The part that holds two or more objective lenses and can be rotated to easily change power (magnification). The revolving nosepiece is a crucial part of the microscope that gives the user the ability to easily change magnification levels simply by rotating the nosepiece. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Objective: The lenses closest to the specimen, that are typically of varying magnifications (e.g., 4x, 10x, 40x, 100x). The different objective lenses allow the user to view the specimen at different levels of magnification. The objective lenses are the lenses closest to the specimen being observed. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Stage: The flat platform where the slide is placed for observation. The stage is a fundamental part of the microscope that enables the user to properly prepare, position, and focus on the specimen they wish to observe and study. It's a critical component for getting high-quality, detailed images through the microscope. Parts of a Microscope and their functions
C. Developing and Deepening Understanding Diaphragm (Iris): A rotating disk under the stage with different sized holes. It is used to vary the intensity and size of the cone of light that is projected upward into the slide. Parts of a Microscope and their functions Illuminator: A light source located at the base of the microscope.
C. Developing and Deepening Understanding Base: The bottom part of the microscope that provides stability and support. It houses the illuminator and other electrical components. The base is the foundation that the rest of the microscope structure is built upon. Its sturdy design helps keep the microscope steady and prevents it from tipping or moving during use. Parts of a Microscope and their functions
C. Developing and Deepening Understanding A. Preparing a Wet Mount Slide Placing the specimen Use a clean microscope slide Add a small drop of water or mounting medium to the center of the slide Carefully place the specimen (e.g. plant tissue, cheek cells) into the drop Microscope Operation and Techniques
C. Developing and Deepening Understanding A. Preparing a Wet Mount Slide 2. Adding a coverslip Gently lower a coverslip at a 45-degree angle onto the drop. Avoid trapping air bubbles under the coverslip. Microscope Operation and Techniques
C. Developing and Deepening Understanding B. Adjusting the Focus Using coarse and fine adjustment knobs. Start with the lowest power objective lens Use the coarse adjustment knob to quickly bring the specimen into view Switch to the fine adjustment knob to refine and sharpen the focus Microscope Operation and Techniques
C. Developing and Deepening Understanding B. Adjusting the Focus 2. Achieving optimal sharpness Observe the specimen and make small, incremental turns of the fine adjustment Continue adjusting until the image is as clear and detailed as possible. Microscope Operation and Techniques
C. Developing and Deepening Understanding C. Controlling Illumination Adjusting the light source brightness Use the diaphragm or light intensity control to adjust the brightness Aim for even, consistent illumination across the field of view Microscope Operation and Techniques
C. Developing and Deepening Understanding C. Controlling Illumination 2. Using the diaphragm or condenser The diaphragm controls the size of the light beam. The condenser lens focuses the light onto the specimen. Adjust these components to optimize contrast and clarity Microscope Operation and Techniques
D. MAKING GENERALIZATION Reflection on Learning One Minute Paper: Using the graphic organizer, reflect on your learning by creating a one-minute paper by answering the questions.
E. EVALUATING LEARNING E. EVALUATING LEARNING E. EVALUATING LEARNING Labelling. Identify the parts of the microscope by writing your answer on the blank provided.
E. EVALUATING LEARNING E. EVALUATING LEARNING E. EVALUATING LEARNING
Reference : SCIENCE 7 LESSON EXEMPLAR Textbooks: "Textbooks on microscopy or general biology: "Microscopy and Histology" by Louie C. Kerr "Fundamentals of Light Microscopy and Electronic Imaging" by Douglas B. Murphy Scientific journals and publications: "Microscopy and Microanalysis" (published by Cambridge University Press) "Journal of Microscopy" (published by Wiley-Blackwell) Educational websites and resources: Microscope manufacturer websites (e.g. Leica, Nikon, Olympus) Science education portals like Science Direct, Khan Academy, or Exploratorium Government or academic institution resources: Microscope operation guides from university biology departments Instructional materials from science museums or national labs