Question Bank – Karp’s Cell and Molecular Biology (9th Edition) by Karp and Iwasa

This Question Bank is designed for Cell and Molecular Biology (9th Edition) by Karp and Iwasa. It includes MCQs, true/false, fill-in-the-blanks, and short-answer questions ideal for quizzes and exams.

1. Package Title: Test Bank
Course Title: Karp9e
Chapter Number: 8



Question Type: Multiple Choice



1) When electron micrographs were first taken of the cell interior, what kinds of structures were seen?

a) membrane-bound vesicles of varying diameter, containing material of different electron density
b) long channels bounded by membranes and radiating through the cytoplasm
c) an interconnected network of canals
d) stacks of flattened, membrane-bound sacs called cisternae
e) all of these are correct

Answer: e

Difficulty: Easy
Learning Objective: LO 8.1 Compare the components of the biosynthetic and endocytic pathways.
Section Reference: Section 8.1 An Overview of the Endomembrane System



Question Type: Multiple Choice



2) The correct order of passage of materials produced in a biosynthetic pathway is:

a) endoplasmic reticulum, Golgi complex, plasma membrane, secretory vesicle
b) endoplasmic reticulum, Golgi complex, secretory vesicle, plasma membrane
c) endoplasmic reticulum, lysosome, secretory vesicle, plasma membrane
d) endoplasmic reticulum, lysosome, Golgi complex, plasma membrane

Answer: b

Difficulty: Hard
Learning Objective: LO 8.1 Compare the components of the biosynthetic and endocytic pathways.
Section Reference: Section 8.1 An Overview of the Endomembrane System



Question Type: Multiple Choice
[email protected]
WhatsApp: https://wa.me/message/2H3BV2L5TTSUF1 - Telegram: https://t.me/solutionmanual
You can access complete document on following URL. Contact me if site not loaded
Contact me in order to access the whole complete document - Email: [email protected]
https://solumanu.com/product/karps-cell-and-molecular-biology/

2. 3) Production and transport of materials in secretory vesicles which occurs continuously is known as:

a) regulated secretion
b) endogenous secretion
c) exogenous secretion
d) constitutive secretion

Answer: d

Difficulty: Easy
Learning Objective: LO 8.1 Compare the components of the biosynthetic and endocytic pathways.
Section Reference: Section 8.1 An Overview of the Endomembrane System



Question Type: Multiple Choice



4) What is the name for a brief incubation of a tissue with radioactivity during which labeled amino acids are
incorporated into protein?

a) chase
b) pulse
c) pulse-chase
d) labelation

Answer: b

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



5) A tissue has been briefly labeled with radiolabeled amino acids. It is then transferred to a medium containing
unlabeled amino acids. This can be done several times with different tissue samples for varying periods of time. What
is the entire procedure called?

3. a) chase
b) pulse
c) pulse-chase
d) labelard
e) statin

Answer: c

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



6) In a pulse-chase procedure, if the chase is longer, which statement below correctly describes the location of the
radioactively labeled proteins in the cell?

a) closer to the synthesis site
b) farther from the nucleus
c) farther from the synthesis site
d) closer to the nucleus
e) farther from the mitonchondria

Answer: c

Difficulty: Medium
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



7) A scientist wishes to visualize movement of a protein in a living cell. Which procedure would work best?
a) pulse-chase
b) fusion of the green fluorescent protein gene to the protein that is to be tracked through the cell
c) fusion of the green fluorescent protein gene to the gene encoding the protein to be tracked through the cell
d) pulse-chase using fluorescent antibodies
e) all of these would work well

4. Answer: c

Difficulty: Medium
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



8) Cells are infected with a vesicular stomatitis virus (VSV) strain in which a viral gene (VSVG) is fused to the green
fluorescent protein gene. When the chimeric protein is synthesized, what pathway does it follow from synthesis until it
leaves the cell?

a) RER, Golgi complex, plasma membrane, viral envelopes
b) RER, Golgi complex, viral envelopes, plasma membrane
c) Golgi complex, RER, plasma membrane, viral envelopes
d) RER, Golgi complex, mitochondria, plasma membrane, viral envelopes
e) RER, mitochondria, Golgi complex, plasma membrane, viral envelopes

Answer: a

Difficulty: Hard
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



9) Cells are infected with a virus carrying a temperature-sensitive mutant VSVG gene that encodes a protein that cannot
leave the ER of infected cells grown at restrictive temperatures (40
o
C). Thus, at higher temperatures:

a) VSVG protein heads immediately for the Golgi complex.
b) VSVG protein cannot leave the ER.
c) VSVG protein leaves the ER immediately.
d) all of the manufactured VSVG protein leaves the ER synchronously.
e) VSVG protein is degraded rapidly and never passes to the Golgi complex.

Answer: b

Difficulty: Hard

5. Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



10) Elevated temperatures at which temperature-sensitive mutants do not work are called ________ temperatures.

a) restrictive
b) permissive
c) temperature-sensitive
d) frame-shift
e) point

Answer: a

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



11) When cells are homogenized, the cytomembrane system is broken into fragments, which can fuse to form small
membranous spheres called ________.

a) vacuoles
b) victuals
c) vesicles
d) nuclei
e) endosomes

Answer: c

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes

6. Question Type: Multiple Choice



12) The separation of organelles or vesicles derived from different organelles is called ______.

a) cell division
b) mitosis
c) meiosis
d) subcellular fractionation
e) cell ostentation

Answer: d

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



13) When homogenized, the endomembrane system is broken up into vesicles, which are heterogeneous but similar in
size. These vesicles can be purified and, after purification, often retain their biological activity. They are collectively
referred to as _________.

a) endosomes
b) microsomes
c) ribosomes
d) minisomes
e) lysosomes

Answer: b

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice

7. 14) Studies of cell physiology that occur in test tubes that do not contain whole cells are called:

a) in vivo systems
b) cell-free systems
c) test tube systems
d) onsite systems
e) cellonic systems

Answer: b

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



15) Why are yeast cells often used to study eukaryotic gene mutations affecting secretion and other cytomembrane
processes?

a) They have only a few genes.
b) They are small and single-celled and can be cultured easily.
c) They can be grown as haploid organisms so mutants are easily seen.
d) Deficiencies in yeast cells caused by mutants are easily detected.
e) All of these are correct.

Answer: e

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



16) What is the effect on a yeast cell of the presence of a mutant gene involved in vesicle fusion?

a) The ER shrinks.
b) The nucleus becomes swollen.
c) The Golgi complex expands greatly.

8. d) Cells accumulate expanded ER cisternae.
e) Cells amass an excess number of unfused vesicles.

Answer: e

Difficulty: Medium
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



17) A cellular phenomenon called RNA interference (RNAi) is a process in which cells produce small RNAs called
_________ bind to specific mRNAs and inhibit the translation of these mRNAs into proteins.

a) snRNAs
b) isRNAs
c) mRNAs
d) RNAsi
e) siRNAs

Answer: e

Difficulty: Easy
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



18) A control cell that is synthesizing a GFP-labeled version of mannosidase II has fluorescence localized in the Golgi
complexes of the cell. Normally, this enzyme is synthesized in the endoplasmic reticulum and moves via transport
vesicles to the Golgi complex, where it takes up residence. If an experimental cell contains an siRNA that leads to the
fluorescence being restricted to the endoplasmic reticulum, with what would the siRNA be likely to interfere?

a) an mRNA that codes for a protein involved in the transport of the enzyme from the ER to the Golgi complex
b) an rRNA that synthesizes the enzyme
c) the synthesis of mannosidase II from its mRNA
d) an mRNA that codes for a protein involved in the transport of the enzyme from the Golgi complex to the ER
e) an mRNA that codes for the enzyme

9. Answer: a

Difficulty: Hard
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



19) What allows smooth and rough vesicles (microsomes) to be readily separated by density gradient centrifugation?

a) their size differences
b) their differences in lipid composition
c) their differences in color
d) their differences in density
e) their differences in water content

Answer: d

Difficulty: Medium
Learning Objective: LO 8.2 Describe five approaches used to study the endomembrane system.
Section Reference: Section 8.2 A Few Approaches to the Study of Endomembranes



Question Type: Multiple Choice



20) What can be used to distinguish RER from SER?
a) the location of the ER
b) the proximity of the ER to the nucleus
c) the protein make-up of the ER
d) the shape of its component lipids


Answer: c

Difficulty: Medium
Learning Objective: LO 8.3 Compare the structures and functions of the RER and SER, and their roles in the
maintenance of cellular proteins and membranes.
Section Reference: Section 8.3 The Endoplasmic Reticulum

10. Question Type: Multiple Choice



21) RER has ribosomes, similarly to which structure that it is continuous with?
a) the inner membrane of the nuclear envelope
b) the outer membrane of the nuclear envelope
c) the outer mitochondrial membrane
d) the outer chloroplast membrane
e) the Golgi complex

Answer: b

Difficulty: Easy
Learning Objective: LO 8.3 Compare the structures and functions of the RER and SER, and their roles in the
maintenance of cellular proteins and membranes.
Section Reference: Section 8.3 The Endoplasmic Reticulum



Question Type: Multiple Choice



22) Which type of cells below is NOT known for its extensively developed SER?

a) skin cells
b) kidney tubule cells
c) skeletal muscle cells
d) steroid-producing endocrine cells
e) both skeletal muscle cells and kidney tubule cells

Answer: a

Difficulty: Easy
Learning Objective: LO 8.3 Compare the structures and functions of the RER and SER, and their roles in the
maintenance of cellular proteins and membranes.
Section Reference: Section 8.3 The Endoplasmic Reticulum



Question Type: Multiple Choice

11. 23) What determines the function of a cell's smooth endoplasmic reticulum?

a) its lipid content
b) its polynucleotide content
c) its carbohydrate content
d) its protein content
e) its age

Answer: d

Difficulty: Medium
Learning Objective: LO 8.3 Compare the structures and functions of the RER and SER, and their roles in the
maintenance of cellular proteins and membranes.
Section Reference: Section 8.3 The Endoplasmic Reticulum



Question Type: Multiple Choice



24) Which of the following is a function associated with the smooth endoplasmic reticulum in at least some cells?

a) synthesis of steroid hormones
b) detoxification of many organic compounds, like barbiturates and ethanol
c) release of glucose into the bloodstream
d) sequestration of calcium Ca
2+
ions within the cisternal space
e) all of these are correct

Answer: e

Difficulty: Medium
Learning Objective: LO 8.3 Compare the structures and functions of the RER and SER, and their roles in the
maintenance of cellular proteins and membranes.
Section Reference: Section 8.3 The Endoplasmic Reticulum



Question Type: Multiple Choice



25) When the SER detoxifies compounds, what is one known potential problem?
a) The detoxified compounds cause excessive weight gain.

12. b) The detoxified compounds cause excessive weight loss.
c)The detoxified compounds are carcinogeneic.
d) The detoxified compounds denature essential enzymes..
e) Detoxification leads to addiction.

Answer: c

Difficulty: Medium
Learning Objective: LO 8.3 Compare the structures and functions of the RER and SER, and their roles in the
maintenance of cellular proteins and membranes.
Section Reference: Section 8.3 The Endoplasmic Reticulum



Question Type: Multiple Choice



26) What cellular response is triggered by the regulated release of Ca
2+
ions from the SER?

a) skeletal muscle cell contraction
b) secretory vesicle fusion with the plasma membrane
c) release of neurotransmitters from nerve cells
d) release of the contents of the acrosome from the head of a sperm

Answer: a

Difficulty: Medium
Learning Objective: LO 8.3 Compare the structures and functions of the RER and SER, and their roles in the
maintenance of cellular proteins and membranes.
Section Reference: Section 8.3 The Endoplasmic Reticulum



Question Type: Multiple Choice



27) What is the arrangement of organelles in a secretory cell from the basal end to the apical end, an arrangement that
reflects the flow of secretory products from synthesis to discharge?

a) nucleus and RER – SER – Golgi complex – secretory vesicles
b) Golgi complex – nucleus and RER – SER – secretory vesicles
c) nucleus and RER – Golgi complex – SER – secretory vesicles
d) SER – nucleus and RER – Golgi complex – secretory vesicles
e) secretory vesicles – nucleus and RER – SER – Golgi complex