Recombinant monoclonal antibody technology

A review term paper on Recombinant monoclonal antibody technology with basic definition and good visualization.

1. Recombinant Monoclonal Antibody Technology M.Amin Ghavami Fall 2016

2. What is Antibody? Also known as Immunoglobulin Is a large, Y-shaped protein Produced mainly by plasma cells Used by the immune system to identify and neutralize pathogens Recognizes unique antigen

3. What is Antibody? Also known as Immunoglobulin Is a large, Y-shaped protein Produced mainly by plasma cells Used by the immune system to identify and neutralize pathogens Recognizes unique antigen

4. What is Antigen?

5. What is Antigen? Anti body gen eration Antigen is any substance that causes an immune system to produce antibodies against it . Sometimes antigens can be part of the host

6. What is Plasma cells and B cells? They are types of with blood cells

7. The main categories of antibody action: Neutralisation Agglutination Precipitation Complement activation (fixation )

8. The main categories of antibody action: Neutralisation Agglutination Precipitation Complement activation (fixation )

9. Paratope and epitope: Paratope is a part of an antibody which recognizes and binds to an antigen Epitope is the part of an antigen that is recognized by the immune system

10. What is monoclonal antibodies ( mAB )? Are antibodies that are made by identical immune cells

11. Polyclonal antibodies vs. monoclonal antibodies Polyclonal antibodies bind to multiple epitopes and are usually made by several different immune cells lineages

12. Monoclonal antibody produce

13. L imitation requires considerable time Expense Expertise Specialized cell culture facilities relies on the use of animals rejected by the human immune system

14. Recombinant Bringing together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome.

15. Recombinant monoclonal antibody Recombinant DNA ( rDNA ) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome.

16. Application