SEAWEED TISSUE CULTURE TECHNIQUE , FACTORS AFFECTING CULTURE.pptx

Introduction of Seaweed tissue culture, factors affecting tissue culture, Advantage, culture technique

1. SEAWEED TISSUE CULTURE ANNAMALAI UNIVERSITY CAS IN MARINE BIOLOGY MALAY DUYARI 2 nd SEMESTER, COASTAL AQUACULTURE REG- 22060014

2. WHAT IS TISSUE CULTURE Tissue culture, also known as cell culture or in vitro cultivation, is the process of growing cells, tissues, or organs in a laboratory under controlled conditions. It involves the isolation of cells from a plant or animal and then growing them in a nutrient-rich solution in a sterile environment. The cells are usually grown on a solid or liquid medium that contains the necessary nutrients, hormones, and other growth factors needed for their survival and growth. 4/18/2023 Designed by PoweredTemplate.com 2

3. SEAWEED TISSUE CULTURE Seaweed tissue culture is the process of cultivating seaweed cells in a laboratory under controlled conditions. Seaweed tissue culture involves the isolation of seaweed cells from a parent plant and growing them in a nutrient-rich medium that contains the necessary nutrients, hormones, and other growth factors needed for their survival and growth. Seaweed tissue culture is used for a variety of purposes, including the production of high-quality seaweed products, such as food, cosmetics, and pharmaceuticals. 4/18/2023 te.com 3

4. FACTORS THAT AFFECT THE SEAWEED TISSUE CULTURE Source of seaweed : The quality of the seaweed sample is an important factor in the success of tissue culture. The seaweed should be healthy and free from any signs of disease or contamination. Composition of the culture medium : The culture medium should contain all the necessary nutrients, hormones, and growth factors required for seaweed growth and development. The composition of the medium will vary depending on the species of seaweed being cultured. Sterilization : It is important to sterilize all equipment, culture vessels, and culture media to prevent contamination from bacteria, fungi, and other microorganisms. 4/18/2023 Designed by PoweredTemplate.com 4

5. Environmental conditions : The environmental conditions, such as temperature, light, and humidity, need to be carefully controlled to ensure optimal growth and development of the seaweed culture . Harvesting : The timing and method of harvesting will depend on the intended use of the seaweed. For example, if the seaweed is being used for food, it should be harvested before it reaches maturity to ensure maximum nutritional value. 4/18/2023 Designed by PoweredTemplate.com 5

6. PROCESS OF THE CULTURE Collection of seaweed : The first step is to collect a sample of the seaweed that is to be cultured. The sample is usually collected from the wild, but in some cases, it may come from a pre-existing culture . Isolation of cells : The collected seaweed sample is then treated to isolate individual cells. This can be done using enzymatic or mechanical methods to break down the seaweed tissue and release the cells. Preparation of culture medium : A culture medium is prepared that contains all the necessary nutrients, hormones, and growth factors needed for the seaweed cells to grow and divide. The culture medium is sterilized to prevent contamination . 4/18/2023 Designed by PoweredTemplate.com 6

7. Inoculation of cells : The isolated seaweed cells are then introduced into the culture medium, either by suspension or by placing the cells onto the surface of the medium . Incubation : The culture is incubated under controlled environmental conditions, such as temperature, light, and humidity. The incubation period can last for several weeks to several months, depending on the species of seaweed being cultured and the desired outcome . Subculture : Once the seaweed culture has reached a certain size, it can be sub cultured by transferring a portion of the culture to a new culture medium to continue the growth cycle . Harvesting : The harvested seaweed can be processed into various products such as food, pharmaceuticals, and cosmetics . 4/18/2023 Designed by PoweredTemplate.com 7

8. ADVANTAGES Sustainable production : Seaweed tissue culture can be used to produce high-quality seaweed biomass in a sustainable and eco-friendly manner, without the need for traditional agricultural practices that can have a negative impact on the environment. Consistent quality : Seaweed tissue culture allows for the production of seaweed biomass with consistent quality and characteristics, which is important for commercial applications such as food and pharmaceuticals. Genetic improvement : Seaweed tissue culture can be used for genetic improvement and selection of desirable traits, such as disease resistance and increased productivity. 4/18/2023 Designed by PoweredTemplate.com 8

9. Cost-effective : Seaweed tissue culture can be a cost-effective method for the production of high-quality seaweed biomass, as it allows for the efficient use of resources and space. Reduced dependence on wild harvest : Seaweed tissue culture can help reduce the dependence on wild harvest of seaweed, which can help conserve natural resources and reduce the impact of overfishing on marine ecosystems. Diversification of products : Seaweed tissue culture can be used to produce a range of products, including food, cosmetics, and pharmaceuticals, which can help diversify industries and create new economic opportunities. 4/18/2023 Designed by PoweredTemplate.com 9

10. REFERENCES Seaweed Culture and Marine Plant Products edited by Ronald L. Sandland and Claire C. Sandland (2011) Seaweed Tissue Culture: Methods and Applications edited by Katsuhiro Shiraiwa and Se-Kwon Kim (2015) Seaweed Invasions: A Synthesis of Ecological, Economic and Legal Imperatives edited by Craig R. Johnson, Jeffrey T. Wright and Alan T. Critchley (2013) Algal Cultures, Analogues of Bloom and Environmental Change edited by K. R. Timmermans, J. R. G. Davenport and S. Batten (2006) Seaweeds and their Role in Globally Changing Environments edited by Charlene G. W. Carvalho and Zhigang Zhou (2010) 4/18/2023 Designed by PoweredTemplate.com 10

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